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Titolo:
RECOMBINANT CHICKEN INTERFERON - A POTENT ANTIVIRAL AGENT THAT LACKS INTRINSIC MACROPHAGE ACTIVATING FACTOR ACTIVITY
Autore:
SCHULTZ U; KASPERS B; RINDERLE C; SEKELLICK MJ; MARCUS PI; STAEHELI P;
Indirizzi:
UNIV FREIBURG,INST MED MIKROBIOL & HYG,DEPT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MED MIKROBIOL & HYG,DEPT VIROL D-79008 FREIBURG GERMANY UNIV MUNICH,INST TIERPHYSIOL MUNICH GERMANY UNIV CONNECTICUT,DEPT MOLEC & CELL BIOL STORRS CT 06269
Titolo Testata:
European Journal of Immunology
fascicolo: 3, volume: 25, anno: 1995,
pagine: 847 - 851
SICI:
0014-2980(1995)25:3<847:RCI-AP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA; PURIFICATION; INDUCTION; PROTEIN; VIRUSES;
Keywords:
RECOMBINANT INTERFERON; CHICKEN; MACROPHAGE ACTIVATING FACTOR; CYTOKINE; ANTIVIRAL ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
U. Schultz et al., "RECOMBINANT CHICKEN INTERFERON - A POTENT ANTIVIRAL AGENT THAT LACKS INTRINSIC MACROPHAGE ACTIVATING FACTOR ACTIVITY", European Journal of Immunology, 25(3), 1995, pp. 847-851

Abstract

Crude preparations of chicken interferon (ChIFN) from various sourcescontain both antiviral and macrophage-activating factor (MAF) activity. Previous serological data indicated that unlike mammals, birds might express only a single type of IFN in response to viruses and mitogens that exhibits both activities. We have now expressed a complementaryDNA for virus-induced ChIFN in transfected COS cells and in Escherichia coli. Purified recombinant ChIFN is a powerful antiviral agent and has high Mr promoter-inducing activity. However, as the sole agent, recombinant ChIFN lacks MAF activity: it does not induce the secretion of nitric oxide in primary monocyte-derived chicken macrophages. A neutralizing antiserum prepared against cloned ChIFN blocks most of the antiviral and Mr promoter-inducing activity present in preparations of natural ChIFN, but does not inhibit the MAF activity These results demonstrate that chicken cells can be induced to secrete a novel cytokine which probably represents the avian homolog of mammalian IFN-gamma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:46:44