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Titolo:
APPLICATION OF THE POLYMERASE CHAIN-REACTION (PCR) TO QUANTIFY MICRO-METASTASIS IN AN EXPERIMENTAL ANIMAL
Autore:
MATANO S; RYOYAMA K; NAKAMURA S; OKADA G; NOMURA T;
Indirizzi:
KANAZAWA UNIV,CANC RES INST,DEPT MOLEC BIOL,13-1 TAKARA MACHI KANAZAWA ISHIKAWA 920 JAPAN KANAZAWA UNIV,CANC RES INST,DEPT MOLEC BIOL KANAZAWA ISHIKAWA 920 JAPAN KANAZAWA UNIV,SCH MED,CANC RES INST,DEPT INTERNAL MED 3 KANAZAWA ISHIKAWA 920 JAPAN KANAZAWA UNIV,CANC RES INST,DEPT EXP THERAP KANAZAWA ISHIKAWA 920 JAPAN
Titolo Testata:
Cancer letters
fascicolo: 1, volume: 91, anno: 1995,
pagine: 93 - 99
SICI:
0304-3835(1995)91:1<93:AOTPC(>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-HA-RAS; ADHESION RECEPTORS; CELLS; GENE; ONCOGENE; MATRIX;
Keywords:
BALB/C MOUSE; R/MHM-SFME-1 CELL; MICRO-METASTASIS; HUMAN C-HA-RAS SEQUENCE; POLYMERASE CHAIN REACTION (PCR);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
S. Matano et al., "APPLICATION OF THE POLYMERASE CHAIN-REACTION (PCR) TO QUANTIFY MICRO-METASTASIS IN AN EXPERIMENTAL ANIMAL", Cancer letters, 91(1), 1995, pp. 93-99

Abstract

In vitro cultured r/mHM-SFME-1 cells were injected into the hind footpads of Balb/c mice. Metastasis was detected in the lungs of tumor-bearing mice by means of both PCR and histological methods, Primers for the PCR were set to amplify a 128 bp exon-1 sequence of the human c-Ha-ras1 gene which had been introduced into the cells. Resulted PCR bands were densitometorically quantified using a bioimage analyzer, and more than 1 x 10(4) tumor cells were detectable in the mouse lung, The number of tumor cells per lung estimated from the amount of PCR products was 1 x 10(5), 15 x 10(5), 1 x 10(5) and 40 x 10(5) on days 7,14,21 and 28 respectively after the tumor injection. No metastases were histologically observed on days 7 and 14, Then, the possibility of using this model system for evaluation of a treatment against micro-metastases is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:26:09