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Titolo:
REPLICATION FACTOR-C INTERACTS WITH THE C-TERMINAL SIDE OF PROLIFERATING CELL NUCLEAR ANTIGEN
Autore:
MOSSI R; JONSSON ZO; ALLEN BL; HARDIN SH; HUBSCHER U;
Indirizzi:
UNIV ZURICH,INST VET BIOCHEM,WINTERTHURERSTR 190 CH-8057 ZURICH SWITZERLAND UNIV HOUSTON,INST MOL BIOL,DEPT BIOCHEM & BIOPHYS SCI,DEPT BIOL HOUSTON TX 77204 TEXAS A&M UNIV,DEPT BIOL COLLEGE STN TX 77843
Titolo Testata:
The Journal of biological chemistry
fascicolo: 3, volume: 272, anno: 1997,
pagine: 1769 - 1776
SICI:
0021-9258(1997)272:3<1769:RFIWTC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMIAN VIRUS-40 DNA; POLYMERASE-DELTA; ESCHERICHIA-COLI; SLIDING CLAMPS; SACCHAROMYCES-CEREVISIAE; TRIMERIC STRUCTURE; LARGE SUBUNIT; CALF THYMUS; RF-C; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
R. Mossi et al., "REPLICATION FACTOR-C INTERACTS WITH THE C-TERMINAL SIDE OF PROLIFERATING CELL NUCLEAR ANTIGEN", The Journal of biological chemistry, 272(3), 1997, pp. 1769-1776

Abstract

Replication factor C (RF-C) is a heteropentameric protein essential for DNA replication and repair. It is a molecular matchmaker required for loading of proliferating cell nuclear antigen (PCNA) onto double-stranded DNA and, thus, for PCNA-dependent DNA elongation by DNA polymerases delta and epsilon. To elucidate the mode of RF-C binding to the PCNA clamp, modified forms of human PCNA were used that could be P-32-labeled in vitro either at the C or the N terminus. Using a kinase protection assay, we show that the heteropentameric calf thymus RF-C was able to protect the C-terminal region but not the N-terminal region of human PCNA from phosphorylation, suggesting that RF C interacts with the PCNA face at which the C termini are located (C-side). A similar protection profile was obtained with the recently identified PCNA binding region (residues 478-712), but not with the DNA binding region (residues 366-477), of the human RF-C large subunit (Fotedar, R., Mossi, R., Fitzgerald, P., Rousselle, T., Maga, G., Brickner, H., Messner, H., Khastilba, S., Hubscher, U., and Fotedar, A., (1996) EMBO J., 15, 4423-4433). Furthermore, we show that the RF-C 36 kDa subunit of human RF-C could interact independently with the C side of PCNA. The RF-C largesubunit from a third species, namely Drosophila melanogaster, interacted similarly with the modified human PCNA, indicating that the interaction between RF C and PCNA is conserved through eukaryotic evolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 17:27:10