Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TERTIARY STRUCTURE OF [2FE-2S] FERREDOXIN FROM SPIRULINA-PLATENSIS REFINED AT 2.5 ANGSTROM RESOLUTION - STRUCTURAL COMPARISONS OF PLANT-TYPE FERREDOXINS AND AN ELECTROSTATIC POTENTIAL ANALYSIS
Autore:
FUKUYAMA K; UEKI N; NAKAMURA H; TSUKIHARA T; MATSUBARA H;
Indirizzi:
OSAKA UNIV,FAC SCI,DEPT BIOL TOYONAKA OSAKA 560 JAPAN PROT ENGN RES INST SUITA OSAKA 565 JAPAN UNIV TOKUSHIMA,FAC ENGN,DEPT BIOL SCI & TECHNOL TOKUSHIMA 770 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 5, volume: 117, anno: 1995,
pagine: 1017 - 1023
SICI:
0021-924X(1995)117:5<1017:TSO[FF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHTHALATE DIOXYGENASE REDUCTASE; NADP+ REDUCTASE; CYTOCHROME-C; PROTEINS; COMPLEX; ANABAENA-7120; FLAVODOXIN; RESIDUES; BINDING; FAMILY;
Keywords:
ELECTROSTATIC POTENTIAL; [2FE-2S] FERREDOXIN; STRUCTURAL COMPARISON; X-RAY STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
K. Fukuyama et al., "TERTIARY STRUCTURE OF [2FE-2S] FERREDOXIN FROM SPIRULINA-PLATENSIS REFINED AT 2.5 ANGSTROM RESOLUTION - STRUCTURAL COMPARISONS OF PLANT-TYPE FERREDOXINS AND AN ELECTROSTATIC POTENTIAL ANALYSIS", Journal of Biochemistry, 117(5), 1995, pp. 1017-1023

Abstract

The structure of plant-type [2Fe-2S] ferredoxin isolated from Spirulina platensis has been refined using diffraction data to 2.5 Angstrom resolution by alternate cycles of simulated annealing and manual revision of the model, The final R factor is 19.9% for 2,912 reflections with F > 2(sigma F) between 8.0 and 2.5 Angstrom resolution, S, platensisferredoxin, like other plant-type [2Fe-2S] ferredoxins, has a major alpha-helix flanking a sheet consisting of four beta strands, The present refinement revises the conformation of residues 56-71, in which a one-turn helix was identified. Superposition of the Spirulina ferredoxin structure on the structures of other ferredoxins that have been wellrefined showed structural perturbation at a few residues on the aminoand carboxyl termini and the turn between the first and second beta-strands, The root-mean-square deviations of the corresponding C-alpha atoms of the pairs of ferredoxins range from 0.90 to 1.17 Angstrom for all the residues, but from 0.64 to 0.70 Angstrom if the few perturbed residues are excluded, Therefore, it may be concluded that the main-chain foldings of all the plant-type [2Fe-2S] ferredoxins are essentially the same, Electrostatic potential analysis showed that the molecularsurface around the cluster is negatively charged, whereas that of thebeta-sheet of the other side is positively charged, The interaction between ferredoxin and ferredoxin-NADP(+) reductase is discussed on thebasis of the charge distributions of these molecules and biochemical data.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:50:33