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Titolo:
EFFECT OF ENHANCER SEQUENCES ON VENTRICULAR MYOSIN LIGHT CHAIN-2 PROMOTER ACTIVITY IN HEART-MUSCLE AND NONMUSCLE CELLS
Autore:
JIN Y; PASUMARTHI KBS; BOCK ME; CHEN YJ; KARDAMI E; CATTINI PA;
Indirizzi:
UNIV MANITOBA,DEPT PHYSIOL,730 WILLIAM AVE WINNIPEG MB R3E 3J7 CANADA UNIV MANITOBA,DEPT PHYSIOL WINNIPEG MB R3E 3J7 CANADA ST BONIFACE GEN HOSP,RES CTR,DIV CARDIOVASC SCI WINNIPEG MB R2H 2A6 CANADA
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 210, anno: 1995,
pagine: 260 - 266
SICI:
0006-291X(1995)210:2<260:EOESOV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT MYOCARDIAL-CELLS; GENE-EXPRESSION; FIREFLY LUCIFERASE; TRANSGENIC MICE; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
Y. Jin et al., "EFFECT OF ENHANCER SEQUENCES ON VENTRICULAR MYOSIN LIGHT CHAIN-2 PROMOTER ACTIVITY IN HEART-MUSCLE AND NONMUSCLE CELLS", Biochemical and biophysical research communications, 210(2), 1995, pp. 260-266

Abstract

Positive (HF-1 and HF-2) and negative (HF-3) elements responsible forcardiac-specificity of the rat ventricular myosin light chain-2 (MLC-2v) promoter are contained in a 250 base pair region. The effect of the simian virus 40 (SV40) enhancer or 3 copies of the HF-1, HF-2 and HF-3 elements on MLC-2v promoter activity and specificity was assessed in neonatal rat cardiac myocytes and cardiac nonmuscle cells as well. as rat heart myoblast H9c2 and glioma (nonmuscle) C6 cell Lines. The SV40 enhancer increased promoter activity by at least 10-fold in both muscle and nonmuscle cell types; however, there was a decrease in cardiac ventricular myocyte-specificity. In contrast, the 3 copies of HF-1, HF-2 and HF-3 elements stimulated MLC-2v promoter activity similar to 3-fold in neonatal ventricular cardiac myocytes alone and, effectively, displayed about a 5-fold increase in specificity over the wild type MLC-2v promoter. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:37:40