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Titolo:
CATALYTIC PROPERTIES OF PHE41-]HIS MUTANT OF HORSERADISH-PEROXIDASE EXPRESSED IN ESCHERICHIA-COLI
Autore:
LOGINOV DB; GAZARYAN IG; DOSEEVA VV; GALKIN AG; TISHKOV VI; MAREEVA EA; ORLOVA MA;
Indirizzi:
MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,LENINSKIE GORY MOSCOW 119899RUSSIA
Titolo Testata:
Russian chemical bulletin
fascicolo: 11, volume: 43, anno: 1994,
pagine: 1923 - 1927
SICI:
1066-5285(1994)43:11<1923:CPOPMO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-C PEROXIDASE; -> VAL; PROTEIN; ACID);
Keywords:
RECOMBINANT HORSERADISH PEROXIDASE; MUTAGENESIS; KINETICS OF ENZYMATIC OXIDATION; RADIATION INACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
D.B. Loginov et al., "CATALYTIC PROPERTIES OF PHE41-]HIS MUTANT OF HORSERADISH-PEROXIDASE EXPRESSED IN ESCHERICHIA-COLI", Russian chemical bulletin, 43(11), 1994, pp. 1923-1927

Abstract

The recombinant horseradish peroxidase and its single-point F41H mutant have been reactivated from E. coli inclusion bodies. The influence of the mutation on the heme entrapment, stability and activity of the enzyme was demonstrated. The catalytic rate constants for H2O2 cleavage and ammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) oxidation decrease by two and one orders of magnitude, respectively. Unlike the wild-type recombinant horseradish peroxidase, the elimination of the ABTS oxidation product is not a rate-determining step for the mutant. The F41H replacement results in significant changes of kinetics of iodide ion oxidation. The reaction rate is linear to the concentrations of iodide, H2O2, and the enzyme. The results suggest the direct interaction of iodide with the porphyrin ring of the heme. The decrease in ABTS oxidation activity accompanied by retention of activity in iodide oxidation in the course of low-dosage radiolysis of the F41Hmutant is additional evidence of the direct electron transfer from iodide to the heme, in contrast to ABTS oxidation, in which the electrontransfer chain in the protein molecule is involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:20:43