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Titolo:
INFECTION OF HUMAN FETAL ASTROCYTES WITH HIV-1 - VIRAL TROPISM AND THE ROLE OF CELL-TO-CELL CONTACT IN VIRAL TRANSMISSION
Autore:
NATH A; HARTLOPER V; FURER M; FOWKE KR;
Indirizzi:
UNIV MANITOBA,DEPT MED MICROBIOL,NEUROVIROL LAB,523-730 WILLIAM AVE WINNIPEG MB R3E 0W3 CANADA
Titolo Testata:
Journal of neuropathology and experimental neurology
fascicolo: 3, volume: 54, anno: 1995,
pagine: 320 - 330
SICI:
0022-3069(1995)54:3<320:IOHFAW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; IMMUNE-DEFICIENCY-SYNDROME; AIDS DEMENTIA COMPLEX; HUMAN GLIAL-CELLS; HUMAN-BRAIN-CELLS; PROGRESSIVE ENCEPHALOPATHY; NUCLEOTIDE-SEQUENCE; TYPE-1 INFECTION; MICROGLIAL CELLS; NERVOUS-SYSTEM;
Keywords:
ADHESION; ASTROCYTES; BRAIN; FETAL; HIV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
A. Nath et al., "INFECTION OF HUMAN FETAL ASTROCYTES WITH HIV-1 - VIRAL TROPISM AND THE ROLE OF CELL-TO-CELL CONTACT IN VIRAL TRANSMISSION", Journal of neuropathology and experimental neurology, 54(3), 1995, pp. 320-330

Abstract

Astrocyte cultures from human fetal brain were infected with human immunodeficiency virus (HIV) either as free virus or with a chronically infected lymphoblastoid cell line and monitored for signs of infection. The lymphocytotropic strains HIV3B and HIVSF2(ARV-2) but not the monocytotropic strain HIVAda-M infected the human fetal astrocytes. The infected cells were monitored by immunocytochemistry, detection of p24 antigen in the supernatants and polymerase chain reaction amplification of the proviral DNA. No morphological or cytopathic effects were seen in these cells. Upon co-curture of astrocytes with a lymphoblastoid cell line chronically infected with HIVSF2(ARV-2), the lymphoblastoid cells readily adhered to the astrocytes as determined by a Cr-51 adhesion assay and by light and electron microscopy. This cell to cell contact resulted in infection of increased numbers of astrocytes. Similar adhesion of lymphoblasts to microglia was not seen. Thus, astrocytes from human fetal brain can be infected in vitro directly by lymphocytotropic strains of HIV or by adherence to infected lymphoblastoid cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 16:36:23