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Titolo:
HIGH-RESOLUTION DNA FIBER-FISH FOR GENOMIC DNA MAPPING AND COLOR BAR-CODING OF LARGE GENES
Autore:
FLORIJN RJ; BONDEN LAJ; VROLIJK H; WIEGANT J; VAANDRAGER JW; BAAS F; DENDUNNEN JT; TANKE HJ; VANOMMEN GJB; RAAP AK;
Indirizzi:
LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 ALLEIDEN NETHERLANDS LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY 2333 AL LEIDEN NETHERLANDS LEIDEN STATE UNIV,DEPT HUMAN GENET 2333 AL LEIDEN NETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,DEPT NEUROL 1105 AZ AMSTERDAM NETHERLANDS
Titolo Testata:
Human molecular genetics
fascicolo: 5, volume: 4, anno: 1995,
pagine: 831 - 836
SICI:
0964-6906(1995)4:5<831:HDFFGD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; DIGITAL IMAGING MICROSCOPY; DMD-GENE; CELL-NUCLEI; INTERPHASE; MULTIPLE; PROBES; DISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Florijn et al., "HIGH-RESOLUTION DNA FIBER-FISH FOR GENOMIC DNA MAPPING AND COLOR BAR-CODING OF LARGE GENES", Human molecular genetics, 4(5), 1995, pp. 831-836

Abstract

We have applied two-colour fluorescence in situ hybridization (FISH) to DNA fibers and combined it with digital imaging microscopy for the mapping of large cosmid contigs. The technique was validated using a set of unique plasmids and a cosmid contig both originating from the thyroglobulin (Tg) gene and previously mapped by restriction analysis. The resolution proved to be close to the theoretical lower limit of similar to 1 kb, ranging greater than or equal to 400 kb. Subsequently a 400 kb cosmid contig derived from a DMD-YAC was directly mapped by Fiber-FISH, The resulting map is in full agreement with the restriction map. Two-colour Fiber-FISH mapping thus showed to be capable for accurately sizing gaps and overlaps, and to identify chimeric or repeat sequence containing cosmids across a 400 kb region at once. The generated 400 kb 'colour bar-code' was subsequently used to map two DMD deletionbreakpoints in patient DNA with an accuracy of 1-2 kb, The results underscore the value of this method for the delineation of chromosomal rearrangements for positional cloning and single patient clinical studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:39:03