Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INVOLVEMENT OF ORNITHINE DECARBOXYLASE AND POLYAMINES IN GLUCOCORTICOID-INDUCED APOPTOSIS OF RAT THYMOCYTES
Autore:
DESIDERIO MA; GRASSILLI E; BELLESIA E; SALOMONI P; FRANCESCHI C;
Indirizzi:
UNIV MILAN,INST GEN PATHOL,VIA MANGIAGALLI 31 I-20133 MILAN ITALY UNIV MILAN,CTR RES CELL PATHOL,CNR I-20133 MILAN ITALY UNIV MODENA,DEPT BIOMED SCI,GEN PATHOL SECT I-41100 MODENA ITALY
Titolo Testata:
Cell growth & differentiation
fascicolo: 5, volume: 6, anno: 1995,
pagine: 505 - 513
SICI:
1044-9523(1995)6:5<505:IOODAP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERMIDINE SPERMINE N1-ACETYLTRANSFERASE; DEXAMETHASONE-INDUCED APOPTOSIS; A-INDUCED PROLIFERATION; PROGRAMMED CELL-DEATH; HISTONE ACETYLTRANSFERASE; GENE-EXPRESSION; MESSENGER-RNA; TRANSFORMATION; LYMPHOCYTES; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Desiderio et al., "INVOLVEMENT OF ORNITHINE DECARBOXYLASE AND POLYAMINES IN GLUCOCORTICOID-INDUCED APOPTOSIS OF RAT THYMOCYTES", Cell growth & differentiation, 6(5), 1995, pp. 505-513

Abstract

Ornithine decarboxylase (ODC), the first and rate-limiting enzyme of polyamine metabolism, has been shown to be required for entry into andprogression through the cell cycle. However, the role of ODC and polyamines in apoptosis remains to be determined. We have examined ODC expression and polyamine levels in thymocytes activated to undergo apoptosis by dexamethasone treatment. We have demonstrated a rapid and reversible induction of ODC (mRNA and activity), as previously reported forthe mRNA expression of other ''early'' genes, c-fos, c-jun, and c-myc, in the same experimental model. Surprisingly, polyamine levels diminished progressively starting at 2-4 h after dexamethasone treatment, and spermine was depleted at 8-12 h. This seemed to be relevant since increasing the intracellular polyamine levels by exogenous spermine administration prevented the DNA ''laddering'' (2-4 h) and the DNA loss from the nucleus (8-18 h) due to dexamethasone treatment. Moreover, theactivities of spermidine/spermine N-1-acetyltransferase, which controls the cytosolic polyamine interconversion pathway, and of spermidine N-8-acetyltransferase, which regulates the nuclear pool and functions of polyamines, were measured in apoptotic cells. Spermidine/spermine N-1-acetyltransferase activity progressively increased and might be responsible for spermidine and spermine excretion as acetyl derivatives. In contrast, spermidine N-8-acetyltransferase activity remained unchanged. A completely different scenario was observed in proliferating concanavalin A-treated thymocytes, studied for comparison. In this case, polyamine levels increased, remaining at high values until 12 h. This is likely a consequence of the rapid and prolonged induction of ODC (mRNA and activity), accompanied by that of spermidine/spermine N-1-acetyltransferase (mRNA and activity). Spermidine N-8-acetyltransferase activity peaked biphasically, indicating its possible involvement in theenhanced gene expression necessary for cell proliferation. Thus, the decrease in intracellular polyamine levels may represent a signal to thymocytes for progression into the apoptotic program.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:48:21