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Titolo:
DYE SCREENING AND SIGNAL-TO-NOISE RATIO FOR RETROGRADELY TRANSPORTED VOLTAGE-SENSITIVE DYES
Autore:
TSAU Y; WENNER P; ODONOVAN MJ; COHEN LB; LOEW LM; WUSKELL JP;
Indirizzi:
GEORGETOWN UNIV,GEORGETOWN INST COGNIT & COMPUTAT SCI WASHINGTON DC 20057 YALE UNIV,SCH MED,DEPT PHYSIOL NEW HAVEN CT 06510 NIH,NEURAL CONTROL LAB,NINDS BETHESDA MD 20892 UNIV CONNECTICUT,CTR HLTH,DEPT PHYSIOL FARMINGTON CT 06032 MARINE BIOL LAB WOODS HOLE MA 02543
Titolo Testata:
Journal of neuroscience methods
fascicolo: 2, volume: 70, anno: 1996,
pagine: 121 - 129
SICI:
0165-0270(1996)70:2<121:DSASRF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHARGE-SHIFT PROBES; SALAMANDER OLFACTORY-BULB; CENTRAL NERVOUS-SYSTEM; MEMBRANE; FLUORESCENCE; NEURONS; AXONS;
Keywords:
VOLTAGE-SENSITIVE DYE; OPTICAL RECORDING; EMBRYONIC CHICK SPINAL CORD; MULTISITE RECORDING; RETROGRADE STAINING; STYRYL DYE; DYE SYNTHESIS; HYDROPHOBIC VS HYDROPHILIC DYE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
Y. Tsau et al., "DYE SCREENING AND SIGNAL-TO-NOISE RATIO FOR RETROGRADELY TRANSPORTED VOLTAGE-SENSITIVE DYES", Journal of neuroscience methods, 70(2), 1996, pp. 121-129

Abstract

Using a novel method for retrogradely labeling specific neuronal populations, we tested different styryl dyes in attempt to find dyes whosestaining would be specific, rapid, and lead to large activity dependent signals. The dyes were injected into the ventral roots of the isolated chick spinal cord from embryos at days E9-E12. The voltage-sensitive dye signals were recorded from synaptically activated motoneurons using a 464 element photodiode array. The best labeling and optical signals were obtained using the relatively hydrophobic dyes di-8-ANEPPQ and di-12-ANEPEQ. Over the 24 h period we examined, these dyes bound specifically to the cells with axons in the ventral roots. The dyes responded with an increase in fluorescence of 1-3% (Delta F/F) in responseto synaptic depolarization of the motoneurons. The signal-to-noise ratio obtained in a single trial from a detector that received light from a 14 X 14 mu m(2) area of the motoneuron population was about 10:1. Nonetheless, signals on neighboring diodes were similar, suggesting that we were not detecting the activity of individual neurons. Retrograde labeling and optical recording with voltage-sensitive dyes provides a means for monitoring the activity of identified neurons in situations where microelectrode recordings are not feasible.

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Documento generato il 28/09/20 alle ore 22:08:39