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Titolo:
SYNERGISTIC ACTION OF VASOPRESSIN AND ALDOSTERONE ON BASOLATERAL NA-K+-ATPASE IN THE CORTICAL COLLECTING DUCT()
Autore:
COUTRY N; FARMAN N; BONVALET JP; BLOTCHABAUD M;
Indirizzi:
FAC XAVIER BICHAT,INSERM,U426,IFR CELLULES EPITHELIALES,BP 416-16 RUEHENRI HUCHARD F-75870 PARIS 18 FRANCE FAC XAVIER BICHAT,INSERM,U426,IFR CELLULES EPITHELIALES F-75870 PARIS18 FRANCE
Titolo Testata:
The Journal of membrane biology
fascicolo: 1, volume: 145, anno: 1995,
pagine: 99 - 106
SICI:
0022-2631(1995)145:1<99:SAOVAA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RABBIT NEPHRON; CELL-VOLUME; SODIUM-TRANSPORT; ION-TRANSPORT; RAT-KIDNEY; TUBULE; AVP; DEOXYCORTICOSTERONE; PGE2; CAMP;
Keywords:
MINERALOCORTICOIDS; MOUSE KIDNEY; OUABAIN BINDING; RUBIDIUM UPTAKE; SODIUM TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
N. Coutry et al., "SYNERGISTIC ACTION OF VASOPRESSIN AND ALDOSTERONE ON BASOLATERAL NA-K+-ATPASE IN THE CORTICAL COLLECTING DUCT()", The Journal of membrane biology, 145(1), 1995, pp. 99-106

Abstract

The respective effects of aldosterone and arginine vasopressin (AVP) were examined on the number of active Na+-K+-ATPase and their pumping activity in nonperfused microdissected mouse cortical collecting tubules (CCD) by measuring specific H-3-ouabain binding and ouabain-sensitive Rb-86 uptake. In adrenalectomized (ADX) animals, incubation of CCD with AVP (10(-8) M for 5 min) had no effect on the number of pumps. Incontrast, in ADX animals replete with aldosterone, AVP induced a congruent to 40% increase in the number of pumps. This was accompanied by a congruent to 60-65% increase in ouabain-sensitive Rb uptake. AVP effect was dose-dependent (10-(10)-10(-8) M) and was reproduced by dDAVP,forskolin and 8-Br cAMP, indicating a V-2 pathway. It was inhibited by amiloride 10(-5) M, and did not occur in CCD incubated in hyperosmotic solution, suggesting that the signal was transmitted via apical sodium entry and cell swelling. Finally, the AVP-dependent increase in the number of pumps was rapid (within 5 min) and transient (<25 min). These results demonstrate that, in the CCD, aldosterone and AVP act synergistically to increase not only the Na+-K+- apical sodium entry but also me basolateral Na+-K+-ATPase transport capacity: AVP allows a rapid recruitment and/or activation of an aldosterone-dependent pool of latent Na+-K+-ATPase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:28:42