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Titolo:
DIRECT MICRODISSECTION AND MICROCLONING OF A TRANSLOCATION BREAKPOINTREGION, T(1-11) (Q42.2-Q21), ASSOCIATED WITH SCHIZOPHRENIA
Autore:
MUIR WJ; GOSDEN CM; BROOKES AJ; FANTES J; EVANS KL; MAGUIRE SM; STEVENSON B; BOYLE S; BLACKWOOD DHR; STCLAIR DM; PORTEOUS DJ; WEITH A;
Indirizzi:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,CREWE RD EDINBURGH EH4 2XU MIDLOTHIAN SCOTLAND UNIV EDINBURGH,ROYAL EDINBURGH HOSP,DEPT PSYCHIAT EDINBURGH EH10 5HF MIDLOTHIAN SCOTLAND RES INST MOLEC PATHOL A-1030 VIENNA AUSTRIA
Titolo Testata:
Cytogenetics and cell genetics
fascicolo: 1-2, volume: 70, anno: 1995,
pagine: 35 - 40
SICI:
0301-0171(1995)70:1-2<35:DMAMOA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSYCHIATRIC-DISORDERS; POLYTENE CHROMOSOMES; LINKAGE; MARKERS; CLONING; GENE; SEQUENCES; DNA; IDENTIFICATION; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
W.J. Muir et al., "DIRECT MICRODISSECTION AND MICROCLONING OF A TRANSLOCATION BREAKPOINTREGION, T(1-11) (Q42.2-Q21), ASSOCIATED WITH SCHIZOPHRENIA", Cytogenetics and cell genetics, 70(1-2), 1995, pp. 35-40

Abstract

We describe the generation of large-fragment microclone libraries from the chromosomal breakpoint of a reciprocal balanced translocation linked to schizophrenia. The abnormality was visible under the phase-contrast microscope, allowing direct dissection from unstained, unbanded metaphases. Two separate microdissection experiments yielded 443 and 672 recombinants, respectively. Following complete EcoRI digestion, inserts with an average size of 0.3 kb (range, 0.2-3 kb) were obtained inthe first experiment and 1.5 kb (range, 0.15-6.5 kb) in the second. FISH analysis of pooled clones ''painted'' back onto the derivative chromosome and assignment of microclones to somatic cell hybrids confirmed the fidelity of the method. Microdissection of chromosome regions identified by karyotype rearrangements in unstained, unbanded metaphasesis a potentially powerful tool for positional cloning.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 05:51:24