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Titolo:
B-CELL EPITOPE MAPPING WITHIN THE MA16 ANTIGENIC SEQUENCE FOUND IN EIMERIA-ACERVULINA MEROZOITES AND SPOROZOITES
Autore:
OLORCAIN P; TALEBI A; MULCAHY G;
Indirizzi:
NATL UNIV IRELAND UNIV COLL DUBLIN,DEPT VET MICROBIOL & PARASITOL,SHELBOURNE RD DUBLIN 4 IRELAND
Titolo Testata:
Veterinary parasitology
fascicolo: 3-4, volume: 66, anno: 1996,
pagine: 147 - 157
SICI:
0304-4017(1996)66:3-4<147:BEMWTM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ANTIGEN; PROTEINS; CHICKENS; FLEXIBILITY; COCCIDIOSIS; PREDICTION; VACCINE; TENELLA;
Keywords:
E-ACERVULINA; B-CELL EPITOPE MAPPING; ANTIGENICITY; PREDICTIVE ALGORITHMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
P. Olorcain et al., "B-CELL EPITOPE MAPPING WITHIN THE MA16 ANTIGENIC SEQUENCE FOUND IN EIMERIA-ACERVULINA MEROZOITES AND SPOROZOITES", Veterinary parasitology, 66(3-4), 1996, pp. 147-157

Abstract

Overlapping heptapeptides derived from the MA16 Eimeria acervulina antigenic sequence (Castle et al., 1991) were synthesised on polypropylene pins ('pepskan' technique, Cambridge Research Biochemicals, UK), Binding of antibodies from chickens and rabbits infected and immunised respectively with various species of Eimeria oocysts (E. acervulina, E.tenella, E praecox, E. necatrix and E. maxima), was examined using the coated pins as the solid phase of an enzyme immunoassay (EIA). Antigenicity of the overlapping synthetic heptapeptides was then analysed using a number of algorithms based on the amino acid sequence to predict secondary protein structure, hydrophilicity, acrophilicity and chainflexibility profiles, The antigenicity of this sequence appears to bequite different from that found for the E. tenella GX3264 antigenic sequence (Bhogal et al., 1992) whose profile was similarly examined (Talebi and Mulcahy, 1994) using the same rabbit and chicken anti-Eimeriaoocyst sera.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 07:16:22