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Titolo:
DNASE I HYPERSENSITIVITY MAPPING AND PROMOTER POLYMORPHISM ANALYSIS OF HUMAN C4
Autore:
VAISHNAW AK; HARGREAVES R; CAMPBELL RD; MORLEY BJ; WALPORT MJ;
Indirizzi:
HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT MED,RHEUMATOL UNIT,CU CANE RD LONDON W12 0NN ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT MED,RHEUMATOL UNIT LONDON W12 0NN ENGLAND UNIV OXFORD,DEPT BIOCHEM,MRC,IMMUNOCHEM UNIT OXFORD OX1 3QU ENGLAND
Titolo Testata:
Immunogenetics
fascicolo: 6, volume: 41, anno: 1995,
pagine: 354 - 358
SICI:
0093-7711(1995)41:6<354:DIHMAP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; REGULATORY REGION; JAPANESE PATIENTS; NULL ALLELES; SLP GENES; MOUSE; C-4; VARIANT; LOCUS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A.K. Vaishnaw et al., "DNASE I HYPERSENSITIVITY MAPPING AND PROMOTER POLYMORPHISM ANALYSIS OF HUMAN C4", Immunogenetics, 41(6), 1995, pp. 354-358

Abstract

Human complement component C4 is encoded by two structurally distinctloci in the major histocompatibility complex (MHC) class III region. The two isotypes, C4A and C4B, differ at only four residues in the C4dfragment, but C4 constitutes the most polymorphic of the complement components. It is not known, however, whether the regions involved in the regulation of C4 expression also display polymorphic variation. By using the technique of DNase I hypersensitivity mapping, we established that the only area of transcriptional activity for C4 in the hepatocyte cell Line, HepG2, occurs approximatly 500 base pairs upstream of the transcriptional start site. This region was found to be remarkably constant in sequence when analyzed in the context of differing MHC haplotypes including HLA B57, C4A6, C4B1, DR7, which has been correlated with reduced expression of the C4A isotpye. Similarly, polymerase chain reaction followed by single-strand conformation polymorphism analysis failed to demonstrate any promoter polymorphisms in 103 individuals comprising 52 systemic lupus erythematosus patients and 51 healthy controls.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:51:36