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Titolo:
THE HIV-1 V3 DOMAIN ON FIELD ISOLATES - PARTICIPATION IN GENERATION OF ESCAPE VIRUS IN-VIVO AND ACCESSIBILITY TO NEUTRALIZING ANTIBODIES
Autore:
ARENDRUP M; AKERBLOM L; HEEGAARD PMH; NIELSON JO; HANSEN JES;
Indirizzi:
UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT INFECT DIS 144 DK-2650 HVIDOVRE DENMARK NATL VET INST,CTR BIOMED,DEPT VIROL S-75007 UPPSALA SWEDEN UNIV COPENHAGEN,PANUM INST,PROT LAB DK-2200 COPENHAGEN DENMARK
Titolo Testata:
Archives of virology
fascicolo: 4, volume: 140, anno: 1995,
pagine: 655 - 670
SICI:
0304-8608(1995)140:4<655:THVDOF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; 3RD VARIABLE DOMAIN; ENVELOPE GLYCOPROTEIN; MONOCLONAL-ANTIBODIES; BIOLOGICAL PROPERTIES; ANTIGENIC VARIATION; SYNTHETIC PEPTIDES; TYPE-1; INFECTION; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M. Arendrup et al., "THE HIV-1 V3 DOMAIN ON FIELD ISOLATES - PARTICIPATION IN GENERATION OF ESCAPE VIRUS IN-VIVO AND ACCESSIBILITY TO NEUTRALIZING ANTIBODIES", Archives of virology, 140(4), 1995, pp. 655-670

Abstract

The V3 domain is highly variable and induces HIV neutralizing antibodies (NA). Here we addressed the issues of 1) the participation of mutations in V3 in generation of neutralization resistant escape virus in vivo and 2) the applicability of synthetic V3 peptides corresponding to field isolates to induce neutralizing immune sera. Seven peptides corresponding to the V3 region of primary and escape virus from 3 HIV-1 infected patients were synthesized and used for antibody (Abs) studiesand immunizations. The anti-V3 Abs titre in patient serum was generally low against peptides corresponding to autologous virus isolated later than the serum sample in contrast to the titre against peptides corresponding to virus isolated earlier than the serum sample. Furthermore, neutralizing anti-V3 monoclonal antibodies (MAbs) raised against V3peptides from laboratory strains of HIV-1 showed distinct binding patterns against V3 peptides corresponding to sequential primary and escape field isolates, with the strongest reactivity against late isolatedescape virus. These observations suggest that the neutralization epitope was influenced by the appearance of mutations. When used as immunogen in rabbits, V3 peptides corresponding to field isolates were highly immunogenic but failed to induce neutralizing or gp120-precipitatingAbs. On the contrary, V3 peptide corresponding to the laboratory strain HXB2 induced HIV neutralizing, gp120-precipitating immune serum. Inconclusion, these data suggest a participation of the V3 domain in the immunoselection of escape virus, and that V3 on early field virus isless accessible to NA than that on laboratory strains.

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Documento generato il 02/12/20 alle ore 15:32:29