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Titolo:
APPLICATION OF DNA-FINGERPRINTING WITH DIGOXIGENATED OLIGONUCLEOTIDE PROBE (CAC)(5) TO ANALYSIS OF THE GENETIC-VARIATION WITHIN TAENIA-TAENIAEFORMIS
Autore:
OKAMOTO M; UEDA H; HAYASHI M; OKU Y; KUROSAWA T; KAMIYA M;
Indirizzi:
OSAKA UNIV,SCH MED,INST EXPTL ANIM SCI,2-2 YAMADAOKA SUITA OSAKA 565 JAPAN HOKKAIDO UNIV,FAC VET MED,ANIM SCI LAB SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Journal of veterinary medical science
fascicolo: 2, volume: 57, anno: 1995,
pagine: 267 - 272
SICI:
0916-7250(1995)57:2<267:AODWDO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCES; HYBRIDIZATION;
Keywords:
(CAC)5; DIGOXIGENATED OLIGONUCLEOTIDE PROBE; DNA FINGERPRINTING; INTRASPECIFIC VARIATION; TAENIA TAENIAEFORMIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M. Okamoto et al., "APPLICATION OF DNA-FINGERPRINTING WITH DIGOXIGENATED OLIGONUCLEOTIDE PROBE (CAC)(5) TO ANALYSIS OF THE GENETIC-VARIATION WITHIN TAENIA-TAENIAEFORMIS", Journal of veterinary medical science, 57(2), 1995, pp. 267-272

Abstract

DNA from T. taeniaeformis digested with the restriction endonuclease was hybridized with digoxigenated oligonucleotide probe (CAC)(5). Metacestode and adult showed same clear multibanding patterns, which were characteristic of multilocus DNA fingerprinting. The fingerprinting patterns were quite different from those of the rodent hosts. Genetic variations in 4 laboratory-reared isolates of T. taeniaeformis, including 3 isolates which have been reported to be indistinguishable by infectivity, morphology and protein composition of metacestode, were investigated using this technique. Each of the 4 isolates exhibited isolate-specific fingerprinting patterns and were easily distinguished from one another, thus it was considered that (CAC)(5) was a highly resolvable and informative probe for cestodes. However, it was also indicated that (CAC)(5) was so sensitive that applying fingerprinting with (CAC)(5) to taxonomical or phylogenetic analysis was limited where habitat of the host was restricted to the small area. in comparison to fingerprinting with P-32-labeled (CAC)(5), fingerprinting with digoxigenated (CAC)(5) represented more and sharper bands. It was considered that a digoxigenated probe was more useful for genetic analysis of cestodes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:20:00