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Titolo:
FLOW CYTOMETRIC ANALYSIS AND IMMUNOHISTOCHEMISTRY OF DELAYED-TYPE HYPERSENSITIVITY RESPONSES IN MICE IMMUNIZED WITH RAT HEPATOCYTES
Autore:
TANABE S; TAURA Y; FURUSAWA S; HIROTA Y; TANAKA M; YOKOSUKA M; KONDO K; MAMBA K; NAKAICHI M; NAKAMA S;
Indirizzi:
YAMAGUCHI UNIV,FAC AGR,DEPT VET SURG YAMAGUCHI 753 JAPAN NATL INST ANIM HLTH,IMMUNE CYTOL LAB IBARAKI 305 OSAKA JAPAN TOKYO UNIV AGR & TECHNOL,VET REPROD LAB TOKYO 183 JAPAN YAMAGUCHI UNIV,FAC AGR,DEPT VET ANAT YAMAGUCHI 753 JAPAN
Titolo Testata:
Journal of veterinary medical science
fascicolo: 2, volume: 57, anno: 1995,
pagine: 245 - 249
SICI:
0916-7250(1995)57:2<245:FCAAIO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
REJECTION; SKIN;
Keywords:
DTH RESPONSE; FLOW CYTOMETRIC ANALYSIS; IMMUNOHISTOCHEMISTRY; XENOHEPATOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
S. Tanabe et al., "FLOW CYTOMETRIC ANALYSIS AND IMMUNOHISTOCHEMISTRY OF DELAYED-TYPE HYPERSENSITIVITY RESPONSES IN MICE IMMUNIZED WITH RAT HEPATOCYTES", Journal of veterinary medical science, 57(2), 1995, pp. 245-249

Abstract

Delayed-type hypersensitivity (DTH) responses to rat hepatocytes (HCs) in mice were investigated by flow cytometric analysis and immunohistochemistry with monoclonal antibodies directed against murine class II, CD4, and CD8 antigens. Mice were immunized subcutaneously (s.c.) with 10(6) rat HCs (referred to as s.c.-immunized mice), and control micewere injected s.c. with sterile Hanks' solution (non-immunized mice). Four days later, 10(5) rat HCs were injected into the footpad of s.c.-immunized mice and non-immunized mice. The DTH response in s.c.-immunized mice significantly increased after challenge when compared to that in non-immunized mice. The numbers of class II+, CD4(+), and CD8(+) cells in the footpad, and CD4(+) and CD8(+) cells in the inguinal lymph node of s.c.-immunized mice significantly increased during the DTH response. An increase in the number of CD4(+) cells in the footpad of s.c.-immunized mice after challenge was more significant than that of non-immunized mice. The number of CD4(+) cells increased more markedly in the footpad of s.c.-immunized mice as compared to that of CD8(+) cells. Furthermore, immunohistochemical studies of the footpad of s.c.-immunized mice showed more severe infiltration of CD4(+) cells rather than CD8(+) cells at the injection site of rat HCs. These results suggest that the DTH response in the footpad of mice immunized with rat HCsis associated with severe infiltration of CD4(+) cells.

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Documento generato il 30/11/20 alle ore 07:07:49