Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
C-13-NMR DETERMINATION OF THE SOLUBILITY AND MOLECULAR-DYNAMICS OF CHOLESTERYL OLEATE IN DIMYRISTOYLPHOSPHATIDYLCHOLINE VESICLES
Autore:
MALCOLMSON RJ; HIGINBOTHAM J; BESWICK PH; REED D; PARKINSON JA; SADLER IH;
Indirizzi:
NAPIER UNIV,DEPT APPL CHEM & PHYS SCI,10 COLINTON RD EDINBURGH EH10 5DT MIDLOTHIAN SCOTLAND NAPIER UNIV,DEPT APPL CHEM & PHYS SCI EDINBURGH EH10 5DT MIDLOTHIAN SCOTLAND NAPIER UNIV,DEPT BIOL SCI EDINBURGH EH10 5DT MIDLOTHIAN SCOTLAND UNIV EDINBURGH,DEPT CHEM EDINBURGH EH9 3JJ MIDLOTHIAN SCOTLAND
Titolo Testata:
Chemistry and physics of lipids
fascicolo: 2, volume: 84, anno: 1996,
pagine: 93 - 104
SICI:
0009-3084(1996)84:2<93:CDOTSA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; EGG PHOSPHATIDYLCHOLINE VESICLES; MODEL-FREE APPROACH; MEMBRANES; ESTERS; NMR; MACROMOLECULES; LOCALIZATION; CONFORMATION; RELAXATION;
Keywords:
C-13-NMR; MOLECULAR DYNAMICS; CHOLESTEROL; DMPC; MEMBRANES; CHOLESTERYL OLEATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Malcolmson et al., "C-13-NMR DETERMINATION OF THE SOLUBILITY AND MOLECULAR-DYNAMICS OF CHOLESTERYL OLEATE IN DIMYRISTOYLPHOSPHATIDYLCHOLINE VESICLES", Chemistry and physics of lipids, 84(2), 1996, pp. 93-104

Abstract

Using C-13-NMR measurements of T-1, T-2 and the nuclear Overhauser enhancement factor at 50.29, 90.55 and 150.87 MHz, we have measured the dynamics of 1.5 mol% cholesteryl oleate in dimyristoylphosphatidylcholine (DMPC) small unilamellar vesicles (SUVs) at 37 degrees C. Using the model-free approach of Lipari and Szabo [J. Am. Chem. Sec. 104: 4546-4559, 1982], we have found that the motion of the rigid steroid ring can be described by an equal contribution from two effective motions with correlation times of 15 and 0.38 ns. The motions of the C-26 and C-27 carbon atoms of cholesteryl oleate were found to have an effectivecorrelation time of 7 +/- 2 ps and a value for the square of the generalised order parameter of 0.03 +/- 0.01. The corresponding values forthe C-25 carbon atom were 14 +/- 9 ps and 0.17 +/- 0.05, showing slower motion and greater order for this carbon atom, which is nearer to the rigid steroid ring. This establishes that the motion of the steroidring of cholesteryl oleate in DMPC vesicles is significantly different to that previously measured for cholesterol in DMPC vesicles. On theother hand, the effective motion of the portions of the correspondingalkyl chains about the steroid ring is the same. On preparing vesicles, when the ester was at the limit of miscibility with the DMPC, the solubility of cholesteryl oleate in DMPC vesicles at 55 degrees C was found to be 2.8 +/- 0.3 mol%. Using aqueous Mn2+ as a paramagnetic relaxation enhancement agent, comparison of the appropriate T-1 values indicated that the carbonyl group of the cholesteryl ester was the same distance from the aqueous phase as the C-4 carbon of the steroid ring. Copyright (C) 1996 Elsevier Science Ireland Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 15:46:38