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Titolo:
REPRESSION OF THE C-JUN TRANSACTIVATION FUNCTION BY THE ADENOVIRUS-TYPE-12 E1A 52R PROTEIN CORRELATES WITH THE INHIBITION OF PHOSPHORYLATION OF THE C-JUN ACTIVATION DOMAIN
Autore:
BROCKMANN D; BURY C; KRONER G; KIRCH HC; ESCHE H;
Indirizzi:
UNIV ESSEN GESAMTHSCH,INST MOLEC BIOL CANC RES,HUFELANDSTR 55 D-45122ESSEN GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 18, volume: 270, anno: 1995,
pagine: 10754 - 10763
SICI:
0021-9258(1995)270:18<10754:ROTCTF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING; MESSENGER-RNAS; GENE-PRODUCT; NEGATIVE REGULATOR; LEUCINE ZIPPERS; ACTING FACTOR; AP-1 ACTIVITY; PRIMARY-CELLS; 5 DNA; FOS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
D. Brockmann et al., "REPRESSION OF THE C-JUN TRANSACTIVATION FUNCTION BY THE ADENOVIRUS-TYPE-12 E1A 52R PROTEIN CORRELATES WITH THE INHIBITION OF PHOSPHORYLATION OF THE C-JUN ACTIVATION DOMAIN", The Journal of biological chemistry, 270(18), 1995, pp. 10754-10763

Abstract

The early region 1A 52R polypeptide, a protein expressed exclusively by the in vivo oncogenic adenovirus subtype 12, represses the trans-activating function of the cellular transcription factor complex AP-1 consisting of c-Jun-c-Jun homodimers. In this report we demonstrate thatthe repression in vivo correlates with a direct physical interaction of the adenovirus protein with c-Jun in vitro. Interestingly, the 52R protein binds to the bZIP domain of c-Jun essential for dimerization and DNA binding but not to the c-dun activation domain. This interaction does not prevent the promoter binding of c-Jun/AP-1. Moreover, the physical association between c-Jun and the TATA box-binding protein TBPis not disturbed by the 52R polypeptide. In fact, we show evidence that down-regulation of c-Jun activity by the adenoviral protein is due to the inhibition of phosphorylation of the c-Jun trans-activation domain. In vivo phosphorylation of the c-Jun activation domain is necessary for the interaction of c-Jun with specific cofactors such as CBP and therefore a prerequisite for the activation of target genes. Due to these results we propose a model in which the 52R protein represses the trans-activating function of c-Jun by preventing its phosphorylationthrough a specific kinase necessary for the activation of the cellular transcription factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:07:20