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Titolo:
ULTRA-SENSITIVE FISH USING PEROXIDASE-MEDIATED DEPOSITION OF BIOTIN-TYRAMIDE OR FLUOROCHROME-TYRAMIDE
Autore:
RAAP AK; VANDECORPUT MPC; VERVENNE RAW; VANGIJLSWIJK RPM; TANKE HJ; WIEGANT J;
Indirizzi:
LEIDEN UNIV,FAC MED,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Human molecular genetics
fascicolo: 4, volume: 4, anno: 1995,
pagine: 529 - 534
SICI:
0964-6906(1995)4:4<529:UFUPDO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; INTRON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
A.K. Raap et al., "ULTRA-SENSITIVE FISH USING PEROXIDASE-MEDIATED DEPOSITION OF BIOTIN-TYRAMIDE OR FLUOROCHROME-TYRAMIDE", Human molecular genetics, 4(4), 1995, pp. 529-534

Abstract

We describe a detection principle for indirect fluorescence in situ hybridization (FISH) methods that with only one or two antibody layers dramatically improves FISH signal intensities, The method uses as a first layer an anti-hapten immunoglobulin [or (strept)avidin] conjugatedto peroxidase, The quintessence of the method is the use of fluorochrome- or biotin labelled tyramides as peroxidase substrates to generateand deposit many fluorochrome or biotin molecules close to the in situ bound peroxidase. These may either be directly evaluated under the fluorescence microscope or after another incubation with fluorochrome-labelled (strept)avidin.

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Documento generato il 28/11/20 alle ore 15:49:27