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Titolo:
IMMUNOLOCALIZATION OF SARCOLEMMAL DIHYDROPYRIDINE RECEPTOR AND SARCOPLASMIC RETICULAR TRIADIN AND RYANODINE RECEPTOR IN RABBIT VENTRICLE AND ATRIUM
Autore:
CARL SL; FELIX K; CASWELL AH; BRANDT NR; BALL WJ; VAGHY PL; MEISSNER G; FERGUSON DG;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT ANAT,10900 EUCLID AVE CLEVELAND OH 44106 UNIV CINCINNATI,DEPT CELLULAR & MOLEC PHYSIOL CINCINNATI OH 45267 UNIV CINCINNATI,DEPT PHARMACOL & CELL BIOPHYS CINCINNATI OH 45267 UNIV MIAMI,DEPT PHARMACOL MIAMI FL 33101 UNIV N CAROLINA,DEPT BIOCHEM CHAPEL HILL NC 27599
Titolo Testata:
The Journal of cell biology
fascicolo: 3, volume: 129, anno: 1995,
pagine: 673 - 682
SICI:
0021-9525(1995)129:3<673:IOSDRA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE CALCIUM-CHANNEL; SKELETAL-MUSCLE; RELEASE CHANNEL; CARDIAC-MUSCLE; ULTRASTRUCTURAL-LOCALIZATION; 1,4-DIHYDROPYRIDINE RECEPTOR; COMPARATIVE STEREOLOGY; FUNCTIONAL EXPRESSION; GLYCOPROTEIN TRIADIN; TRANSVERSE TUBULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Carl et al., "IMMUNOLOCALIZATION OF SARCOLEMMAL DIHYDROPYRIDINE RECEPTOR AND SARCOPLASMIC RETICULAR TRIADIN AND RYANODINE RECEPTOR IN RABBIT VENTRICLE AND ATRIUM", The Journal of cell biology, 129(3), 1995, pp. 673-682

Abstract

The subcellular distribution of sarcolemmal dihydropyridine receptor (DHPR) and sarcoplasmic reticular triadin and Ca2+ release channel/ryanodine receptor (RyR) was determined in adult rabbit ventricle and atrium by double labeling immunofluorescence and laser scanning confocal microscopy. In ventricular muscle cells the immunostaining was observed primarily as transversely oriented punctate bands spaced at approximately 2-mu m intervals along the whole length of the muscle fibers. Image analysis demonstrated a virtually complete overlap of the stainingpatterns of the three proteins, suggesting their close association ator near dyadic couplings that are formed where the sarcoplasmic reticulum (SR) is apposed to the surface membrane or its infoldings, the transverse (T-) tubules. In rabbit atrial cells, which lack an extensiveT-tubular system, DHPR-specific staining was observed to form discrete spots along the sarcolemma but was absent from the interior of the fibers. In atrium, punctate triadin- and RyR-specific staining was alsoobserved as spots at the cell periphery and image analysis indicated that the three proteins were co-localized at, or just below, the sarcolemma. In addition, in the atrial cells triadin- and RyR-specific staining was observed to form transverse bands in the interior cytoplasm at regularly spaced intervals of approximately 2 mu m. Electron microscopy suggested that this cytoplasmic staining was occurring in regions where substantial amounts of extended junctional SR were present. These data indicate that the DHPR codistributes with triadin and the RyR in rabbit ventricle and atrium, and furthermore suggest that some of the SR Ca2+ release channels in atrium may be activated in the absence of a close association with the DHPR.

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Documento generato il 07/07/20 alle ore 12:46:07