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Titolo:
MEASUREMENT BY THE POLYMERASE CHAIN-REACTION OF THE EPSTEIN-BARR-VIRUS LOAD IN INFECTIOUS-MONONUCLEOSIS AND AIDS-RELATED NON-HODGKINS-LYMPHOMAS
Autore:
LAROCHE C; DROUET EB; BROUSSET P; PAIN C; BOIBIEUX A; BIRON F; ICART J; DENOYEL GA; NIVELEAU A;
Indirizzi:
FAC PHARM GRENOBLE,SERV MICROBIOL & IMMUNOL,DOMAINE MERCI F-38706 LA TRONCHE FRANCE INST PASTEUR,UNITE INFECTIOL LYON FRANCE HOP CROIX ROUSSE,MALAD INFECT CLIN F-69317 LYON FRANCE CHU PURPAN,CENT ANATOMOPATHOL LAB TOULOUSE FRANCE CHU RANGUEIL,SERV VIROL F-31054 TOULOUSE FRANCE
Titolo Testata:
Journal of medical virology
fascicolo: 1, volume: 46, anno: 1995,
pagine: 66 - 74
SICI:
0146-6615(1995)46:1<66:MBTPCO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; VIRAL-DNA; LYMPHOPROLIFERATIVE DISEASES; SEROPOSITIVE INDIVIDUALS; HUMAN PAPILLOMAVIRUS; NODE BIOPSIES; CELLS; SEQUENCES; AMPLIFICATION; REPLICATION;
Keywords:
QUANTIFICATION; VIRAL INFECTION; DISEASE; EBV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
C. Laroche et al., "MEASUREMENT BY THE POLYMERASE CHAIN-REACTION OF THE EPSTEIN-BARR-VIRUS LOAD IN INFECTIOUS-MONONUCLEOSIS AND AIDS-RELATED NON-HODGKINS-LYMPHOMAS", Journal of medical virology, 46(1), 1995, pp. 66-74

Abstract

A polymerase chain reaction (PCR) assay for the detection of Epstein-Barr virus (EBV) sequences in various clinical samples, especially peripheral blood leukocytes (PBL) and serum, was carried out and the results obtained were compared with specific EBV serology. One hundred seventy patients were enrolled in the study: 89 healthy blood donors, 22 asymptomatic patients, 36 individuals with primary EBV infection (including 19 patients with infectious mononucleosis [IM]), 22 HIV-infectedsubjects (including 4 with hairy oral leukoplakia, 3 with central nervous disorders, and 15 with non-Hodgkin's lymphoma). All the serum samples from the healthy blood donors were negative. In patients with IM and in AIDS-non Hodgkin's lymphoma (ARNHL), PCR was strongly positive in leukocytes (>2,000 genome equivalents/10(4) cells), which was correlated with detectable amounts of EBV DNA in serum. The overall positivity rate of PCR in serum was 58.8%, 68%, and 73% of cases for non-IM primary EBV infections, IM, and ARNHL, respectively. In two cases of EBV primary infection, the viral DNA was detected in serum, respectively1 month and 2 months before IgM positivity and IgG rise. In one case of ARNHL followed up for several months, PCR (viral load of 2,000 genome equivalents/10(4) cells) became positive concurrently with appearance of lymphoma. In immunocompromised individuals, PCR EBV, if carried out in larger prospective studies, could be considered as a tumor marker, useful for predicting EBV-driven lymphoma and follow-up therapy. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:05:23