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Titolo:
PHARMACOLOGICAL MODEL OF CATECHOLAMINE DEPLETION IN THE HYPOTHALAMUS OF FETAL AND NEONATAL RATS AND ITS APPLICATION
Autore:
BERNABE J; PROSHLYAKOVA E; SAPRONOVA A; TREMBLEAU A; CALAS A; UGRUMOV M;
Indirizzi:
RUSSIAN ACAD SCI,INST DEV BIOL,LAB HORMONAL REGULAT,26 VAVILOV STR MOSCOW 117808 RUSSIA UNIV PARIS 06,CNRS,URA 1488,INST NEUROSCI,LAB PHYSIOL REPROD PARIS FRANCE UNIV PARIS 06,CNRS,URA 1488,INST NEUROSCI,DEPT NEUROBIOL INTERCELLULAR SIGNAL PARIS FRANCE
Titolo Testata:
Cellular and molecular neurobiology
fascicolo: 6, volume: 16, anno: 1996,
pagine: 617 - 624
SICI:
0272-4340(1996)16:6<617:PMOCDI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; TYROSINE-HYDROXYLASE; BRAIN; ONTOGENY; NEURONS; NEUROTRANSMITTERS; DIFFERENTIATION; EXPRESSION; DOPAMINE;
Keywords:
CATECHOLAMINES; HYPOTHALAMUS; DEVELOPMENT; UPTAKE; ALPHA-METHYL-M-TYROSINE; 6-HYDROXYDOPAMINE; HPLC; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
J. Bernabe et al., "PHARMACOLOGICAL MODEL OF CATECHOLAMINE DEPLETION IN THE HYPOTHALAMUS OF FETAL AND NEONATAL RATS AND ITS APPLICATION", Cellular and molecular neurobiology, 16(6), 1996, pp. 617-624

Abstract

1. The present study aimed to develop a pharmacological model of catecholamine (CA) depletion in the hypothalamus during the period of its morphofunctional development, i.e. in fetal and neonatal rats of both sexes. 2. In the first series of experiments, pregnant females and, hence, fetuses were systemically treated daily from the embryonic day (E) 13 to E20 with the inhibitor of the CA synthesis alpha-methyl-m-tyrosine. The CA concentrations;were subsequently measured in the fetal hypothalamus at E21 by high performance liquid chromatography with electrochemical detection (HPLC-ED). In the second series of experiments, neonatal rats were injected with neurotoxin, 6-hydroxydopamine and/or alpha-methyl-m-tyrosine daily from the 2nd postnatal day (P2) to P10. 3. The HPLC-ED assay of hypothalamic catecholamines (CA's) at E21 and P11 showed that both in fetuses and neonates, alpha-methyl-m-tyrosine caused more than 50% depletion of hypothalamic noradrenaline and adrenaline, while the dopamine (DA) level remained unchanged. The combined treatment of neonatal rats with alpha-methyl-m-tyrosine and 6-hydroxydopamine resulted additionally in a 25% decreased level of DA. 4. The influence of CA deficiency on the developing hypothalamic CA system was further evaluated by measuring [H-3]DA uptake by nervous tissue in vitro. 5. The CA deficiency caused a 50% drop of [H-3]DA uptake by the hypothalamic tissue in treated fetuses suggesting a stimulating effect of CA's on the early development of the CA system. In pharmacologicallytreated neonatal rats [H-3]DA uptake remained at the control level showing no influence of the CA deficiency on the developing CA system after birth. 6. The usefulness of the proposed pharmacological model forstudying of CA influence on differentiating hypothalamic target neurons is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:28:44