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Titolo:
CALCIUM OSCILLATIONS AND PROTEIN-SYNTHESIS INHIBITION SYNERGISTICALLYACTIVATE MOUSE OOCYTES
Autore:
BOSMIKICH A; SWANN K; WHITTINGHAM DG;
Indirizzi:
ST GEORGE HOSP,SCH MED,MRC,EXPTL EMBRYOL & TERATOL UNIT,CRANMER TERRACE LONDON SW17 0RE ENGLAND ST GEORGE HOSP,SCH MED,MRC,EXPTL EMBRYOL & TERATOL UNIT LONDON SW17 0RE ENGLAND UNIV COLL LONDON,DEPT ANAT & DEV BIOL LONDON ENGLAND
Titolo Testata:
Molecular reproduction and development
fascicolo: 1, volume: 41, anno: 1995,
pagine: 84 - 90
SICI:
1040-452X(1995)41:1<84:COAPIS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAPHASE-II ARREST; PARTHENOGENETIC ACTIVATION; MEIOTIC MATURATION; MAMMALIAN OOCYTES; BOVINE OOCYTES; EGGS; STIMULATION; CA-2+; FERTILIZATION; PROGRESSION;
Keywords:
ACTIVATION; CALCIUM; STRONTIUM; CYCOHEXIMIDE; OOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
A. Bosmikich et al., "CALCIUM OSCILLATIONS AND PROTEIN-SYNTHESIS INHIBITION SYNERGISTICALLYACTIVATE MOUSE OOCYTES", Molecular reproduction and development, 41(1), 1995, pp. 84-90

Abstract

We have examined the ability of the two parthenogenetic agents, strontium (Sr2+) and cycloheximide, to activate mouse oocytes. We demonstrate that Sr2+ and cycloheximide act synergistically to promote parthenogenetic activation up to the pronuclear stage in oocytes collected immediately after ovulation. These two agents appeared to act independently, since incubation in Sr2+ media triggered a series of intracellularCa2+ rises without affecting protein synthesis and cycloheximide inhibited protein synthesis without causing any intracellular Ca2+ changes. In addition, cycloheximide did not alter the pattern of Ca2+ oscillations induced by Sr2+. In contrast, we show that another commonly usedparthenogenetic activation treatment, the Ca2+ ionophore A23187, has dual effects. Exposure of oocytes to the Ca2+ ionophore, A 23187, in Ca2+- and Mg2+-free medium leads to the activation of young oocytes. However, as well as generating a Ca2+ increase, the treatment of mouse oocytes with A23187 and Ca2+- and Mg2+-free media led to a marked inhibition of protein synthesis. Our data show that parthenogenetic agents may have two important loci for activating mammalian oocytes and that the combined effect on Ca2+ release and protein synthesis is most effective. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:42:47