Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FUNCTIONAL INTERACTIONS BETWEEN THE RETINOBLASTOMA (RB) PROTEIN AND SP-FAMILY MEMBERS - SUPERACTIVATION BY RB REQUIRES AMINO-ACIDS NECESSARY FOR GROWTH SUPPRESSION
Autore:
UDVADIA AJ; TEMPLETON DJ; HOROWITZ JM;
Indirizzi:
DUKE UNIV,MED CTR,DEPT MOLEC CANC BIOL & MICROBIOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT MOLEC CANC BIOL & MICROBIOL DURHAM NC 27710 CASE WESTERN RESERVE UNIV,INST PATHOL CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,CELL BIOL PROGRAM CLEVELAND OH 44106
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 92, anno: 1995,
pagine: 3953 - 3957
SICI:
0027-8424(1995)92:9<3953:FIBTR(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOVIRUS E1A PROTEINS; BOX-BINDING-PROTEINS; LARGE T-ANTIGEN; GENE-PRODUCT; SUSCEPTIBILITY GENE; C-MYC; TRANSCRIPTIONAL REGULATION; EXPRESSION; PROMOTER; DOMAINS;
Keywords:
ANTIONCOGENE; TUMOR-SUPPRESSOR GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Udvadia et al., "FUNCTIONAL INTERACTIONS BETWEEN THE RETINOBLASTOMA (RB) PROTEIN AND SP-FAMILY MEMBERS - SUPERACTIVATION BY RB REQUIRES AMINO-ACIDS NECESSARY FOR GROWTH SUPPRESSION", Proceedings of the National Academy of Sciences of the United Statesof America, 92(9), 1995, pp. 3953-3957

Abstract

The transient expression of the retinoblastoma protein (Rb) regulatesthe transcription of a variety of growth-control genes, including c-fos, c-myc, and the gene for transforming growth factor beta 1 via discrete promoter sequences termed retinoblastoma control elements (RCE), Previous analyses have shown that Sp1 is one of three RCE-binding proteins identified in nuclear extracts and that Rb functionally interactswith Sp1 in vivo, resulting in the ''superactivation'' of Sp1-mediated transcription, By immunochemical and biochemical criteria, we reportthat an Sp1-related transcription factor, Sp3, is a second RCE-binding protein, Furthermore, in transient cotransfection assays, we report that Rb ''superactivates'' Sp3-mediated RCE-dependent transcription invivo and that levels of superactivation are dependent on the trans-activator (Sp1 or Sp3) studied, Using expression vectors carrying mutated Rb cDNAs, we have identified two portions of Rb required for superactivation: (i) a portion of the Rb ''pocket'' (amino acids 614-839) previously determined to be required for physical interactions between Rband transcription factors such as E2F-1 and (ii) a novel amino-terminal region (amino acids 140-202). Since both of these regions of Rb aretargets of mutation in human tumors, our data suggest that superactivation of Sp1/Sp3 may play a role in Rb-mediated growth suppression and/or the induction of differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:00:12