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Titolo:
CYTOSINE-ARABINOSIDE INDUCES APOPTOSIS IN CEREBELLAR NEURONS IN CULTURE
Autore:
DESSI F; POLLARD H; MOREAU J; BENARI Y; CHARRIAUTMARLANGUE C;
Indirizzi:
INSERM,U29,123 BLVD PORT ROYAL F-75014 PARIS FRANCE INSERM,U29 F-75014 PARIS FRANCE
Titolo Testata:
Journal of neurochemistry
fascicolo: 5, volume: 64, anno: 1995,
pagine: 1980 - 1987
SICI:
0022-3042(1995)64:5<1980:CIAICN>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; NERVE GROWTH-FACTOR; KILLS POSTMITOTIC NEURONS; DNA FRAGMENTATION; MOLECULAR MECHANISMS; NUCLEOSIDE TRANSPORT; FACTOR DEPRIVATION; ACUTE-LEUKEMIA; STRAND BREAKS; RAT;
Keywords:
CYTOSINE ARABINOSIDE; DNA FRAGMENTATION; APOPTOTIC CELL DEATH; CEREBELLAR NEURON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
F. Dessi et al., "CYTOSINE-ARABINOSIDE INDUCES APOPTOSIS IN CEREBELLAR NEURONS IN CULTURE", Journal of neurochemistry, 64(5), 1995, pp. 1980-1987

Abstract

Cytosine arabinoside (AraC) is a pyrimidine antimetabolite that prevents cell proliferation by inhibiting DNA synthesis. We report that AraC kills cultured cerebellar neurons in a concentration-dependent fashion with an EC(50) of similar to 60 mu M when added shortly after seeding. This cell death has apoptotic features because we observed (1) morphology of apoptotic nuclei as judged by DNA staining with Hoechst 33258, (2) DNA fragmentation with typical ladder pattern on agarose gel, (3) positive nuclear labeling with a specific in situ DNA fragmentation staining, (4) prevention by deoxycytidine (IC50 = 1 mu M), protein, and RNA synthesis inhibitors, and (5) release of DNA fragments in the incubating medium, We have also observed that several proteins were overexpressed in AraC-treated neurons by two-dimensional polyacrylamide gel electrophoresis. We conclude that AraC induces a signal that triggers a cascade of new mRNA and protein synthesis, leading to apoptotic cell death in cultured cerebellar granule cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:34:21