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Titolo:
HUMAN RIBOSOMAL-RNA GENE-CLUSTER - IDENTIFICATION OF THE PROXIMAL ENDCONTAINING A NOVEL TANDEM REPEAT SEQUENCE
Autore:
SAKAI K; OHTA T; MINOSHIMA S; KUDOH J; WANG YM; DEJONG PJ; SHIMIZU N;
Indirizzi:
KEIO UNIV,SCH MED,DEPT MOLEC BIOL,SHINJUKU KU,35 SHINANOMACHI TOKYO 160 JAPAN KEIO UNIV,SCH MED,DEPT MOLEC BIOL,SHINJUKU KU TOKYO 160 JAPAN ROSWELL PK CANC INST,DEPT HUMAN GENET BUFFALO NY 14263
Titolo Testata:
Genomics
fascicolo: 3, volume: 26, anno: 1995,
pagine: 521 - 526
SICI:
0888-7543(1995)26:3<521:HRG-IO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; HUMAN ACROCENTRIC CHROMOSOMES; ALPHOID REPETITIVE DNA; BETA-SATELLITE DNA; III DNA; SUBFAMILIES; FRACTIONATION; LOCALIZATION; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
K. Sakai et al., "HUMAN RIBOSOMAL-RNA GENE-CLUSTER - IDENTIFICATION OF THE PROXIMAL ENDCONTAINING A NOVEL TANDEM REPEAT SEQUENCE", Genomics, 26(3), 1995, pp. 521-526

Abstract

Human ribosomal RNA genes (rDNA) are arranged as tandem repeat clusters on the short arms of five pairs of acrocentric chromosomes, We havedemonstrated that a majority of the rDNA clusters are,detected as 3-Mb DNA fragments when released from human genomic DNA by EcoRV digestion. This indicated the absence of the EcoRV restriction site within therDNA clusters. We then screened for rDNA-positive cosmid clones usinga chromosome aa-specific cosmid library that was constructed from MboI partial digests of the flow-sorted chromosomes. Three hundred twentyrDNA-positive clones negative for the previously reported distal flanking sequence (pACR1) were chosen and subjected to EcoRV digestion. Seven clones susceptible to EcoRV were further characterized as candidate clones that might have been derived from the junctions of the 3-Mb rDNA cluster. We identified one clone containing part of the rDNA unit sequence and a novel flanking sequence. Detailed analysis of this unique clone revealed that the coding region of the last rRNA gene locatedat the proximal end of the cluster is interrupted with a novel sequence of similar to 147 bp that is tandemly repeated and is connected with an intervening 68-bp unique sequence. This junction sequence was readily amplified from chromosomes 21 and 15 as well as 22 using the polymerase chain reaction. Fluorescence in situ hybridization further indicated that the similar to 147-bp sequence repeat is commonly distributed among all the acrocentric short arms. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:07:20