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Titolo:
NUCLEOSOME POSITIONING BY HUMAN ALU ELEMENTS IN CHROMATIN
Autore:
ENGLANDER EW; HOWARD BH;
Indirizzi:
NICHHD,MOLEC GROWTH REGULAT LAB BETHESDA MD 20892 NICHHD,MOLEC GROWTH REGULAT LAB BETHESDA MD 20892
Titolo Testata:
The Journal of biological chemistry
fascicolo: 17, volume: 270, anno: 1995,
pagine: 10091 - 10096
SICI:
0021-9258(1995)270:17<10091:NPBHAE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-III; REPEATED DNA-SEQUENCES; TUMOR VIRUS PROMOTER; MAMMALIAN GENOMES; TRANSGENIC MICE; XENOPUS-LAEVIS; GLOBIN GENES; TRANSCRIPTION; FAMILY; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
E.W. Englander e B.H. Howard, "NUCLEOSOME POSITIONING BY HUMAN ALU ELEMENTS IN CHROMATIN", The Journal of biological chemistry, 270(17), 1995, pp. 10091-10096

Abstract

Alu sequences are interspersed throughout the genomes of primate cells, occurring singly and in clusters around RNA polymerase II-transcribed genes, Because these repeat elements are capable of positioning nucleosomes in in vitro reconstitutes (Englander, E. W., Wolffe, A. P., and Howard, B. H. (1993) J. Biol. Chem. 268, 19565-19573), we investigated whether they also influence in vivo chromatin structure. When assayed collectively using consensus sequence probes and native chromatin as template, Alu family members were found to confer rotational positioning on nucleosomes or nucleosome-like particles. In particular, a 10-base pair pattern of DNase I nicking that spanned the RNA polymerase III box A promoter motif extended upstream to cover diverse 5'-flanking sequences, suggesting that Alu repeats may in fluence patterns of nucleosome formation over neighboring regions. Computational analysis ofa set of naturally occurring Alu sequences indicated that nucleosome positioning information is intrinsic to these elements, Inasmuch as local chromatin organization influences gene expression, the capacity ofAlu sequences to affect chromatin structure as demonstrated here may help to clarify some features of these elements.

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Documento generato il 29/05/20 alle ore 17:10:52