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Titolo:
THE APPENDAGE DOMAIN OF ALPHA-ADAPTIN IS A HIGH-AFFINITY BINDING-SITEFOR DYNAMIN
Autore:
WANG LH; SUDHOF TC; ANDERSON RGW;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET DALLAS TX 75235 UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST DALLAS TX 75235
Titolo Testata:
The Journal of biological chemistry
fascicolo: 17, volume: 270, anno: 1995,
pagine: 10079 - 10083
SICI:
0021-9258(1995)270:17<10079:TADOAI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLATHRIN-COATED PITS; DROSOPHILA-MELANOGASTER; INVITRO BINDING; AP-2 SUBUNIT; PROTEIN; SHIBIRE; VESICLES; ENDOCYTOSIS; MUTATIONS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
L.H. Wang et al., "THE APPENDAGE DOMAIN OF ALPHA-ADAPTIN IS A HIGH-AFFINITY BINDING-SITEFOR DYNAMIN", The Journal of biological chemistry, 270(17), 1995, pp. 10079-10083

Abstract

Dynamin is a GTPase that appears to be required for endocytosis. Eventhough this molecule is known to be in surface-coated pits, the identity of the resident coat proteins that account for this localization is not known, Here we show that dynamin is one of three synaptic terminal proteins that bind with specificity to the ap pendage domain of alpha-adaptin. Binding is sensitive to both salt and pH levels but is notaffected by nucleotides. Using recombinant dynamin expressed in SF9 cells, we estimate that the binding affinity is similar to 200 nM. Binding does not require GTP, and the GTPase activity of dynamin is not stimulated by this interaction. These results suggest that the COOH terminus of alpha-adaptin may be a domain within AP2 that mediates the initial interactions between dynamin and surface-coated pits, This may bean essential step in the regulation of coated pit budding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 22:08:03