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Titolo:
PROGESTERONE-RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE PRIMATE CORPUS-LUTEUM DURING THE MENSTRUAL-CYCLE - POSSIBLE REGULATION BY PROGESTERONE
Autore:
DUFFY DM; STOUFFER RL;
Indirizzi:
OREGON REG PRIMATE RES CTR,DIV REPROD SCI,505NW 185TH AVE BEAVERTON OR 97006
Titolo Testata:
Endocrinology
fascicolo: 5, volume: 136, anno: 1995,
pagine: 1869 - 1876
SICI:
0013-7227(1995)136:5<1869:PMITPC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTEINIZING-HORMONE SURGE; GRANULOSA-CELLS; RHESUS-MONKEY; ESTROGEN-RECEPTORS; OVULATORY CHANGES; GENE-EXPRESSION; LOCALIZATION; FOLLICLES; REQUIREMENTS; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
D.M. Duffy e R.L. Stouffer, "PROGESTERONE-RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE PRIMATE CORPUS-LUTEUM DURING THE MENSTRUAL-CYCLE - POSSIBLE REGULATION BY PROGESTERONE", Endocrinology, 136(5), 1995, pp. 1869-1876

Abstract

In classical target tissues, progesterone (P) down-regulates its own receptor, yet in the primate corpus luteum, progesterone receptors (PRs) exist within a very high local P milieu. The percentage of luteal cells staining PR-positive by immunocytochemistry is highest at the midluteal phase of the menstrual cycle during the period of peak serum P. To investigate the regulation of luteal PRs, we developed a solution hybridization/ribonuclease protection assay for the analysis of PR messenger RNA (mRNA) in macaque corpora lutea (n = 3-4/group). A 332-basepair fragment of the macaque PR complementary DNA corresponding to thehormone-binding region was used as a template for riboprobe production; the specific hybridization of this riboprobe with PR mRNA was confirmed with Northern analysis. P regulation of luteal PR mRNA was investigated by administering trilostane, a 3 beta-hydroxysteroid dehydrogenase inhibitor, to female rhesus macaques beginning on day 6 or 7 of the luteal phase, which reduced serum P until the time of lutectomy. By 18 h after trilostane treatment, luteal PR mRNA levels were significantly elevated compared to untreated control values (mean +/- SEM, 2.0 +/- 0.4 us. 0.7 +/- 0.3; P < 0.05). Reduction in P levels for 4 days after trilostane administration decreased luteal PR mRNA levels comparedwith control values (0.50 +/- 0.02 vs. 1.1 +/- 0.2; P < 0.05). To characterize changes in PR mRNA during the lifespan of the corpus luteum,mRNA levels in luteal tissues from the early, mid-, mid-late, and late luteal phases were determined. PR mRNA levels were lowest during theearly luteal phase and increased (P < 0.05) 3-fold by the mid-late luteal phase; this higher PR mRNA level was maintained throughout the remainder of the luteal phase. These data indicate that P or a metabolite may acutely regulate primate luteal PR mRNA in a manner consistent with PR regulation in classical P target tissues. In contrast, PR mRNA levels parallel increases in P and PR-positive luteal cells during theearly, mid-, and mid-late portions of the luteal phase. High PR mRNA levels are maintained during luteal regression as P and the percentageof PR-positive cells decline, suggesting that PR and PR mRNA are regulated in an asynchronous manner during the lifespan of the corpus luteum in the menstrual cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:19:44