Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PARP PROMOTER-MEDIATED ACTIVATION OF A VSG EXPRESSION SITE PROMOTER IN INSECT FORM TRYPANOSOMA-BRUCEI
Autore:
URMENYI TP; VANDERPLOEG LHT;
Indirizzi:
MERCK & CO INC,RES LABS,DEPT MOLEC BIOL & GENET,POB 2000 RAHWAY NJ 07065 MERCK & CO INC,RES LABS,DEPT MOLEC BIOL & GENET RAHWAY NJ 07065
Titolo Testata:
Nucleic acids research
fascicolo: 6, volume: 23, anno: 1995,
pagine: 1010 - 1018
SICI:
0305-1048(1995)23:6<1010:PPAOAV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEIN; RNA-POLYMERASE-II; AMANITIN-RESISTANT TRANSCRIPTION; DNA REARRANGEMENT EVENTS; PROTEIN-CODING GENES; C-TERMINAL DOMAIN; AFRICAN TRYPANOSOMES; REPETITIVE PROTEIN; ANTIGENIC VARIATION; LARGEST SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
T.P. Urmenyi e L.H.T. Vanderploeg, "PARP PROMOTER-MEDIATED ACTIVATION OF A VSG EXPRESSION SITE PROMOTER IN INSECT FORM TRYPANOSOMA-BRUCEI", Nucleic acids research, 23(6), 1995, pp. 1010-1018

Abstract

In trypanosomes the rRNA, PARP and VSG gene promoters mediate alpha-amanitin-resistant transcription of protein coding genes, presumably byRNA polymerase (pol) I. We compared the activity of PARP and VSG promoters integrated at one of the alleles of the largest subunit of pol II genes in insect form trypanosomes. Even though both promoters are roughly equally active in transient transformation assays in insect formtrypanosomes, only the PARP promoter functioned effectively when integrated at the pol II largest subunit or other loci. Promoter activity in transient transformation assays is therefore not necessarily predictive of transcriptional activity once integrated into the trypanosome genome. The integrated fully active PARP promoter could upregulate in cis an otherwise poorly active integrated VSG promoter, The PARP promoter nucleotide sequence elements responsible for VSG promoter activation coincided with most of the important PARP promoter elements mapped previously by linker scanning mutagenesis, indicating that it is not asingle unique promoter element that was responsible for VSG promoter activation, The data suggest that PARP promoter-mediated activation ofthe VSG promoter does not result from complementation of the VSG promoter with a single insect form-specific transcription factor whose binding site is missing from the VSG promoter and present in the PARP promoter, We favor a model in which chromatin structure at the locus is altered by the PARP promoter, allowing VSG promoter activation in insect form trypanosomes. We discuss the significance of these observationsfor the control of VSG promoters in insect form trypanosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/12/20 alle ore 00:02:36