Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SPONTANEOUS QUANTAL TRANSMITTER SECRETION FROM MYOCYTES AND FIBROBLASTS - COMPARISON WITH NEURONAL SECRETION
Autore:
GIROD R; POPOV S; ALDER J; ZHENG JQ; LOHOF A; POO MM;
Indirizzi:
COLUMBIA UNIV,DEPT BIOL SCI NEW YORK NY 10027 COLUMBIA UNIV,DEPT BIOL SCI NEW YORK NY 10027
Titolo Testata:
The Journal of neuroscience
fascicolo: 4, volume: 15, anno: 1995,
pagine: 2826 - 2838
SICI:
0270-6474(1995)15:4<2826:SQTSFM>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE-ELECTROPLAQUE JUNCTION; XENOPUS CELL-CULTURE; PROTEIN-KINASE-C; NEUROMUSCULAR-JUNCTION; SYNAPTIC TRANSMISSION; ACETYLCHOLINE-RELEASE; MUSCLE INTERACTIONS; EMBRYONIC NEURONS; PHORBOL ESTERS; MEMBRANE;
Keywords:
QUANTAL SECRETION; ACH; CELL CULTURES; FIBROBLAST; MYOCYTE; XENOPUS; SYNAPTIC TRANSMISSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
R. Girod et al., "SPONTANEOUS QUANTAL TRANSMITTER SECRETION FROM MYOCYTES AND FIBROBLASTS - COMPARISON WITH NEURONAL SECRETION", The Journal of neuroscience, 15(4), 1995, pp. 2826-2838

Abstract

When exogenous ACh is loaded into the cytoplasm of cultured amphibianmyocytes and fibroblasts, the cells undergo spontaneous quantal ACh secretion, as detected by the appearance of pulsatile membrane currentsin Xenopus myocytes which are manipulated into contact with the cells, These currents resemble in many ways the miniature endplate currents(MEPCs) observed at developing neuromuscular synapses formed on theseXenopus myocytes, Analyses of the frequency, amplitude, and time course of these currents suggests similarity in the cellular mechanisms involved in the packaging and secretion of ACh quanta in fibroblasts, myocytes, and developing neurons, The size of the ACh packets released by the non-neuronal cells were found to be very similar to the size of the neuronal ACh quanta, which are thought to result from the exocytotic release of synaptic vesicles, Moreover, the kinetics with which theACh packets are discharged from all three cell types are comparable, although the speed of secretion in non-neuronal cells is somewhat slower and more irregular, The spontaneous quantal ACh secretion from neurons and myocytes was decreased by reducing cytosolic Ca2+ level and enhanced by activation of protein kinase C with phorbol ester, but secretion from fibroblasts was unaffected by both treatments, The spontaneous secretion from fibroblasts did show some sensitivity to a rise in cytosolic Ca2+ after treatment with a Ca2+ ionophore, These observations support the hypothesis that the basic machinery for transmitter secretion operating in neurons derive from a more ubiquitous mechanism used for constitutive secretion and membrane trafficking in non-neuronal cells, and neuronal differentiation involves expression of additional unique components for the regulation of the spontaneous quantal secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:21:56