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Titolo:
CHARACTERISTICS AND REGULATION OF 11 BETA-HYDROXYSTEROID DEHYDROGENASE OF PROXIMAL AND DISTAL NEPHRON
Autore:
ALFAIDY N; BLOTCHABAUD M; ROBIC D; KENOUCH S; BOURBOUZE R; BONVALET JP; FARMAN N;
Indirizzi:
INST FED RECH CELLULES EPITHELIALES,UER X BICHAT,INSERM,U246,BP 416 F-75870 PARIS 18 FRANCE INST FED RECH CELLULES EPITHELIALES,UER X BICHAT,INSERM,U246 F-75870 PARIS 18 FRANCE UNIV PARIS 05,FAC PHARM F-75006 PARIS FRANCE
Titolo Testata:
Biochimica et biophysica acta (G). General subjects
fascicolo: 3, volume: 1243, anno: 1995,
pagine: 461 - 468
SICI:
0304-4165(1995)1243:3<461:CARO1B>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIVER 11-BETA-HYDROXYSTEROID DEHYDROGENASE; BLADDER CELL-LINE; RAT-KIDNEY; CORTICOSTEROID 11-BETA-DEHYDROGENASE; TARGET-CELLS; MINERALOCORTICOID RECEPTOR; DEPENDENT ISOFORM; RABBIT NEPHRON; LOCALIZATION; ALDOSTERONE;
Keywords:
ALDOSTERONE; GLUCOCORTICOIDS; KIDNEY; NAD; NADP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
N. Alfaidy et al., "CHARACTERISTICS AND REGULATION OF 11 BETA-HYDROXYSTEROID DEHYDROGENASE OF PROXIMAL AND DISTAL NEPHRON", Biochimica et biophysica acta (G). General subjects, 1243(3), 1995, pp. 461-468

Abstract

Enzymatic properties of the enzyme 11 beta-hydroxysteroid dehydrogenase (11-HSD), which confers mineralocorticoid selectivity, have been explored in the aldosterone-sensitive collecting duct (CCD) and the aldosterone-insensitive Pars Recta (PR) of the rat kidney. After incubation of freshly isolated tubular segments with [H-3]corticosterone (H-3-B) or [H-3]dehydrocorticosterone (H-3-A), the rate of transformation ofH-3-B into H-3-A (dehydrogenase activity), or the reverse reaction (reductase activity) were measured by HPLC. V-max for dehydrogenase activity was found to be 8- to 10-fold higher in CCD than PR. The enzyme functions over a very wide range (0.1-5000 nM) of corticosterone concentration. In CCD, enzyme kinetics suggest either the presence of two 11-HSD forms, differing by their affinity for corticosterone, or complexkinetics. Addition of NAD or NADP to permeabilized tubules revealed that dehydrogenase activity is NAD-dependent in CCD and NADP-dependent in PR. Cofactor addition was ineffective in intact tubules. CCD exhibited an exclusive dehydrogenase activity, whereas in PR dehydrogenase and reductase activity were found. Na regulation of dehydrogenase activity could be evidenced in adrenalectomized rats receiving or not aldosterone, corticosterone or dexamethasone, for 2 h, 3 days or 4 days. Weconclude that 11-HSD in the CCD and PR differs by its V-max and cofactor dependence. Corticosteroid hormones do not influence 11-HSD activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 20:59:09