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Titolo:
IN-VITRO AND IN-VIVO EFFECTS OF LEAD ON SPECIFIC H-3 PN200-110 BINDING TO DIHYDROPYRIDINE RECEPTORS IN THE FRONTAL-CORTEX OF THE MOUSE-BRAIN
Autore:
SCHULTE S; MULLER WE; FRIEDBERG KD;
Indirizzi:
UNIV HEIDELBERG,FAK KLIN MED MANNHEIM,INST PHARMAKOL & TOXIKOL,MAYBACHSTR 14-16 D-68169 MANNHEIM GERMANY ZENT INST SEEL GESUNDHEIT,PSYCHOPHARMAKOL ABT J5 D-68159 MANNHEIM GERMANY
Titolo Testata:
Toxicology
fascicolo: 1-3, volume: 97, anno: 1995,
pagine: 113 - 121
SICI:
0300-483X(1995)97:1-3<113:IAIEOL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-ANTAGONIST BINDING; RAT-BRAIN; CHANNELS; EXPOSURE; BEHAVIOR; AGONISTS; SITES; MICE;
Keywords:
HEAVY METALS; LEAD POISONING; DHP-RECEPTORS; CALCIUM CHANNELS; NEUROTOXICITY; H-3 PN200-110;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S. Schulte et al., "IN-VITRO AND IN-VIVO EFFECTS OF LEAD ON SPECIFIC H-3 PN200-110 BINDING TO DIHYDROPYRIDINE RECEPTORS IN THE FRONTAL-CORTEX OF THE MOUSE-BRAIN", Toxicology, 97(1-3), 1995, pp. 113-121

Abstract

It is assumed that several neurotoxic substances interfere with neuronal calcium channels. Therefore, we studied the effects of the heavy metals, cadmium, copper, lead, manganese, and zinc on the L-type calcium channels in the mouse brain. Characterization of the calcium channels was carried out using binding studies on homogenates from the frontal cortex with the DHP (dihydropyridine)-derivative, H-3-PN200-110, which binds with high affinity to the DHP-receptor inside the L-type calcium channel. Furthermore, the in vivo effects of lead on the DHP-receptors were investigated in perinatally exposed mice. In these animals, the analysis of saturation experiments with H-3-PN200-110 showed no changes in receptor density or ligand affinity due to the bad exposure. In vitro, H-3-PN200-110 binding is absolutely dependent on the presence of calcium. Divalent cations, such as magnesium or manganese, which normally block the physiological effects of calcium, also enhance DHP-receptor binding. Interestingly, ions such as lead, cadmium and copperstimulate H-3-PN200-110 binding at low concentrations (0.1-10 mu M), but inhibit binding at higher concentrations. In contrast, zinc blocked DHP-receptor binding at low concentrations (<100 mu M) without any stimulating effects. These results suggest that modulation of the L-type calcium channel by heavy metal cations is one possible mechanism by which the regulation of calcium homeostasis in neurons is altered.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:23:52