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Titolo:
EFFECT OF ZOLPIDEM ON GAMMA-AMINOBUTYRIC-ACID (GABA)-INDUCED INHIBITION PREDICTS THE INTERACTION OF ETHANOL WITH GABA ON INDIVIDUAL NEURONSIN SEVERAL RAT-BRAIN REGIONS
Autore:
CRISWELL HE; SIMSON PE; KNAPP DJ; DEVAUD LL; MCCOWN TJ; DUNCAN GE; MORROW AL; BREESE GR;
Indirizzi:
UNIV N CAROLINA,BRAIN & DEV RES CTR,CB 7250 CHAPEL HILL NC 27599 UNIV N CAROLINA,CTR ALCOHOL STUDIES,DEPT PSYCHIAT CHAPEL HILL NC 00000 UNIV N CAROLINA,CTR ALCOHOL STUDIES,DEPT PHARMACOL CHAPEL HILL NC 00000
Titolo Testata:
The Journal of pharmacology and experimental therapeutics
fascicolo: 1, volume: 273, anno: 1995,
pagine: 526 - 536
SICI:
0022-3565(1995)273:1<526:EOZOG(>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBUNIT MESSENGER-RNAS; INDUCED CHLORIDE CURRENTS; SPINAL-CORD NEURONS; A RECEPTOR; ALPHA-SUBUNIT; BENZODIAZEPINE RECEPTORS; FUNCTIONAL EXPRESSION; AUDIOGENIC-SEIZURES; INFERIOR COLLICULUS; GENE-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
81
Recensione:
Indirizzi per estratti:
Citazione:
H.E. Criswell et al., "EFFECT OF ZOLPIDEM ON GAMMA-AMINOBUTYRIC-ACID (GABA)-INDUCED INHIBITION PREDICTS THE INTERACTION OF ETHANOL WITH GABA ON INDIVIDUAL NEURONSIN SEVERAL RAT-BRAIN REGIONS", The Journal of pharmacology and experimental therapeutics, 273(1), 1995, pp. 526-536

Abstract

Previous investigations have suggested a relationship between zolpidem binding within specific brain regions and the ability of ethanol or zolpidem to enhance gamma-aminobutyric acid (GABA)-induced inhibition. The purpose of the present study was to extend our electrophysiological analysis to additional brain sites with high levels of zolpidem binding. In the brain regions chosen, red nucleus and globus pallidus, GABA-induced inhibition was shown to be enhanced by either ethanol or zolpidem on some, but not all, neurons. These findings led to the hypothesis that the effect of zolpidem on GABA-induced inhibition would predict the action of ethanol on responses to GABA for that neuron. When zolpidem and ethanol were applied individually to the same neurons in the red nucleus and globus pallidus, those neurons sensitive to zolpidem enhancement of GABA also were sensitive to ethanol. Conversely, if zolpidem did not enhance responses to GABA, ethanol did not enhance responses to GABA at these brain sites. A similar relationship between the abilities of zolpidem and ethanol to enhance GABA-induced inhibitionwas obtained in 90% of the neurons studied in the medial septum/diagonal band and ventral pallidum. These studies provide further support for the contention that the zolpidem-sensitive GABA(A)-benzodiazepine isoreceptor also responds to ethanol. Finally, the expression of GABA, subunit mRNAs was analyzed by polymerase chain reaction from micropunches of several brain regions that contain zolpidem binding sites and exhibit sensitivity to ethanol. Polymerase chain reaction analysis proved more sensitive than in situ hybridization in the detection of receptor subunit mRNAs. Several subunits (alpha 1, alpha 2, alpha 3, beta 2, beta 3 and gamma 2) were common to all brain regions in which ethanol and zolpidem enhanced GABA responses. GABA(A) receptor alpha 4/5, alpha 6, beta 1, gamma 1, gamma 3 and delta subunits were not consistently expressed in association with the presence of zolpidem binding. These data are consistent with the view that one native GABA(A) receptor to which zolpidem binds, and on which ethanol acts, contains the GABA,receptor subunits alpha(1), beta(2), and gamma(2); however, the present investigation did not preclude the possibility that other subunit combinations can contribute to ethanol and zolpidem enhancement of responses to GABA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/08/20 alle ore 17:48:52