Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CELL-TYPE DIFFERENCES IN GOLGI RETENTION SIGNALS FOR TRANSMEMBRANE PROTEINS
Autore:
TANG BL; LOW SH; WONG SH; HONG WJ;
Indirizzi:
NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB,10 KENT RIDGE CRESCENT SINGAPORE 0511 SINGAPORE NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB SINGAPORE 0511 SINGAPORE
Titolo Testata:
European journal of cell biology
fascicolo: 4, volume: 66, anno: 1995,
pagine: 365 - 374
SICI:
0171-9335(1995)66:4<365:CDIGRS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE; LYSOSOMAL MEMBRANE GLYCOPROTEIN; ENDOPLASMIC-RETICULUM; CYTOPLASMIC DOMAIN; ACID-PHOSPHATASE; PLASMA-MEMBRANE; BREFELDIN-A; MDCK CELLS; TRANSPORT; TAIL;
Keywords:
GOLGI RETENTION; LYSOSOME; CHO CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Tang et al., "CELL-TYPE DIFFERENCES IN GOLGI RETENTION SIGNALS FOR TRANSMEMBRANE PROTEINS", European journal of cell biology, 66(4), 1995, pp. 365-374

Abstract

The transmembrane domain of Golgi resident proteins such as beta-galactoside alpha 2,6-sialyltransferase (ST) and N-acetylglucosaminyltransferase 1 (NT) contain a Golgi retention signal which confers Golgi retention to reporter proteins appended to them in the appropriate context, Thus, chimeras of the cell surface protein dipeptidyl peptidase IV containing the transmembrane domain of ST and NT are retained in the Golgi apparatus in MOCK and COS cells, as assessed by indirect immunofluorescence microscopy. Transfection of these chimeric constructs into CHO cells, however, results in their transport to vesicular structureswhich do not colocalize with that of an endogenous Golgi marker, mannosidase II, Furthermore, the staining pattern of these structures are not affected by brefeldin A. Biochemical analysis of the transgene products in pulse chase experiments revealed that the chimeric proteins eventually become resistant to endoglycosidase H, suggesting that they are transported beyond the medial Golgi and therefore the vesicular structures are likely to be post-Golgi, The vesicular structures colocalized well with a lysosomal marker, cathepsin D, and also with internalized FITC-dextran chased into the Lysosomal compartment, Monitoring the cell surface appearance of the chimeric protein suggests that the majority is transported directly to the lyso somal compartment, Golgi retention can be completely restored for ST and improved for NT by the inclusion of sequences flanking the transmembrane domain, Our results reflect cell type differences in the interpretation of the transmembrane domain Golgi retention signal, established that general Golgi retention of type II glycosyltransferases requires the hydrophilic flanking sequence as web as the transmembrane domain, and demonstrate that proteins which escape Golgi retention may be channeled to the lysosomal pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 22:21:24