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Titolo:
DESIGN OF 4-HELIX BUNDLE PROTEIN AS A CANDIDATE FOR HIV VACCINE
Autore:
EROSHKIN AM; KARGINOVA EA; GILEVA IP; LOMAKIN AS; LEBEDEV LR; KAMYININA TP; PEREBOEV AV; IGNATEV GM;
Indirizzi:
NPO VECTOR,MOLEC BIOL RES INST KOLTSOV 633159 RUSSIA
Titolo Testata:
Protein engineering
fascicolo: 2, volume: 8, anno: 1995,
pagine: 167 - 173
SICI:
0269-2139(1995)8:2<167:DO4BPA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; NUCLEOSIDE H-PHOSPHONATES; SYNTHETIC IMMUNOGEN; ESCHERICHIA-COLI; B-CELL; ENVELOPE; PEPTIDES; DETERMINANTS; CONSTRUCTION; INFECTION;
Keywords:
B CELL EPITOPE; 4-HELIX BUNDLE PROTEIN; HIV VACCINE DESIGN; IMMUNOGENICITY; T CELL EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Eroshkin et al., "DESIGN OF 4-HELIX BUNDLE PROTEIN AS A CANDIDATE FOR HIV VACCINE", Protein engineering, 8(2), 1995, pp. 167-173

Abstract

To be efficient, a synthetic vaccine should contain different T and Bcell epitopes of human immunodeficiency virus (HTV) antigens, and theB epitope regions in the vaccine and in the HIV should be conformationally similar. We have suggested previously the construction of vaccines in the form of a protein with a predetermined tertiary structure, namely a four-alpha-helix bundle. Antigenic determinants of cellular and humoral immunity are blocks for the vaccine design. From experimentally studied HIV-1 T and B cell epitopes, we constructed a sequence of a four-helix protein TBI (($) under bar T and ($) under bar B cell epitopes containing immunogen). The gene of the protein was synthesized and the protein was produced in C600 Escherichia coli cells under recA promoter from Proteus mirabelis. CD spectroscopy of the protein demonstrated that 30% of amino acid residues adopt an alpha-helical conformation. Mice immunized with TBI have shown both humoral and cellular immune responses to HIV-1. The obtained data show that the design of TBI was successful. The synthesized gene structure makes possible further reconstruction and improvement of the protein vaccine structure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:43:54