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Titolo:
DETECTION OF GIARDIA-LAMBLIA CYSTS IN STOOL SAMPLES BY IMMUNOFLUORESCENCE USING MONOCLONAL-ANTIBODY
Autore:
WINIECKAKRUSNELL J; LINDER E;
Indirizzi:
SWEDISH INST INFECT DIS CONTROL,PARASITOL LAB S-10521 STOCKHOLM SWEDEN SWEDISH INST INFECT DIS CONTROL,PARASITOL LAB S-10521 STOCKHOLM SWEDEN KAROLINSKA INST,CTR MICROBIOL & TUMOR BIOL,DEPT CLIN PARASITOL S-10521 STOCKHOLM SWEDEN
Titolo Testata:
European journal of clinical microbiology & infectious diseases
fascicolo: 3, volume: 14, anno: 1995,
pagine: 218 - 222
SICI:
0934-9723(1995)14:3<218:DOGCIS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRESERVED FECAL SPECIMENS; ENTAMOEBA-HISTOLYTICA; INTESTINAL PARASITES; DIAGNOSIS; PROTEINS; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J. Winieckakrusnell e E. Linder, "DETECTION OF GIARDIA-LAMBLIA CYSTS IN STOOL SAMPLES BY IMMUNOFLUORESCENCE USING MONOCLONAL-ANTIBODY", European journal of clinical microbiology & infectious diseases, 14(3), 1995, pp. 218-222

Abstract

The diagnostic potential of indirect immunofluorescence to detect Giardia cysts in stool samples using a cyst-specific anti-Giardia lambliamonoclonal antibody was evaluated in comparison to conventional lightmicroscopy. One hundred fifty specimens from clinically suspected Giardia infections and 50 control samples from microscopically proved Giardia infections were tested, Giardia cysts were found in 15 of 150 (10%) samples tested by light microscopy, whereas immunofluorescence microscopy detected 35 of 150 (23 %) positive samples. Forty-six of the 50 reference samples previously shown to contain Giardia cysts were positive. Apparently, the four discrepant samples contained very low numbers of parasites, as none could be detected by conventional microscopy. The results show that Giardia lamblia cysts are detected significantly more frequently using the antibody marker. The doubled number of positive stool specimens and detection of as little as four cysts per sample suggest that microscopical examination of samples can be improvedby immunofluorescent staining of Giardia lamblia cysts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:53:02