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Titolo:
CONSTRUCTION OF NEISSERIA-MENINGITIDIS STRAINS CARRYING MULTIPLE CHROMOSOMAL COPIES OF THE POR-A GENE FOR USE IN THE PRODUCTION OF A MULTIVALENT OUTER-MEMBRANE VESICLE VACCINE
Autore:
VANDERLEY P; VANDERBIEZEN J; POOLMAN JT;
Indirizzi:
NATL INST PUBL HLTH & ENVIRONM PROTECT,ANTONIE VAN LEEUWENHOEKLAAN 9,POB 1 3720 BA BILTHOVEN NETHERLANDS
Titolo Testata:
Vaccine
fascicolo: 4, volume: 13, anno: 1995,
pagine: 401 - 407
SICI:
0264-410X(1995)13:4<401:CONSCM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
B MENINGOCOCCAL VACCINE; MONOCLONAL-ANTIBODIES; BACTERICIDAL ANTIBODIES; PROTECTIVE EFFICACY; INFANT RAT; PROTEINS; CLASS-1; SEROTYPES; NORWAY; DISEASE;
Keywords:
NEISSERIA MENINGITIDIS; PORA GENE; OUTER MEMBRANE VESICLE VACCINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
P. Vanderley et al., "CONSTRUCTION OF NEISSERIA-MENINGITIDIS STRAINS CARRYING MULTIPLE CHROMOSOMAL COPIES OF THE POR-A GENE FOR USE IN THE PRODUCTION OF A MULTIVALENT OUTER-MEMBRANE VESICLE VACCINE", Vaccine, 13(4), 1995, pp. 401-407

Abstract

Starting with Neisseria meningitidis strain H44/76, a set of strains was constructed for use in production of a multivalent outer membrane vesicle vaccine. The aim was to remove unwanted outer membrane components and at the same rime to improve the range of protection. This was accomplished through transformation with plasmid constructs ucts made in Escherichia coli and their homologous recombination into the meningococcal chromosome. Deletion of the cps locus resulted in loss of expression of the group B capsular polysaccharide as well as the lacto-N-neotetraose structure in lipapolysaccharide. Deletion of the porB gene abolished expression of the class 3 outer membrane protein. Additionalcopies of the porA gene, encoding the immunodominant class 1 outer membrane protein, were inserted into one of the opa genes and into the rmpM gene encoding the class 4 outer membrane protein. This construction was done with three sets of porA alleles, resulting in three trivalent strains, each of which expressed a different combination of class 1epitopes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:02:55