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Titolo:
TECHNICAL NOTE - DETECTION AND QUANTIFICATION OF SUPPLEMENTAL FUNGAL BETA-GLUCANASE ACTIVITY IN ANIMAL FEED
Autore:
WALSH GA; MURPHY RA; KILLEEN GF; HEADON DR; POWER RF;
Indirizzi:
NATL UNIV IRELAND UNIV COLL GALWAY,ALLTECHS EUROPEAN BIOSCI RES CTR GALWAY IRELAND NATL UNIV IRELAND UNIV COLL GALWAY,DEPT BIOCHEM,CELL & MOLEC BIOL GRPGALWAY IRELAND
Titolo Testata:
Journal of animal science
fascicolo: 4, volume: 73, anno: 1995,
pagine: 1074 - 1076
SICI:
0021-8812(1995)73:4<1074:TN-DAQ>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYMES; ASSAY; DIETS;
Keywords:
BETA-GLUCANASE; ASSAYS; FEEDS; MEASUREMENT; DETERMINATION; SUPPLEMENTS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
G.A. Walsh et al., "TECHNICAL NOTE - DETECTION AND QUANTIFICATION OF SUPPLEMENTAL FUNGAL BETA-GLUCANASE ACTIVITY IN ANIMAL FEED", Journal of animal science, 73(4), 1995, pp. 1074-1076

Abstract

Selected hydrolytic enzymes are added to animal feeds in order to degrade specific antinutritional factors and(or) to increase availabilityof certain components of feedstuffs to the animal. A method is described that allows detection and quantification of beta-glucanase activity in complex feedstuffs. The method is based on radial diffusion of anenzyme-containing feed extract through an agar gel in which lichenan substrate (a relatively inexpensive glucan of mixed beta 1-->4 and beta 1-->3 linkages) has been dissolved. A linear relationship between the diameter of the zone of substrate hydrolyzed and the log of enzyme activity present was observed. The assay described is technically straightforward and requires no specialized equipment. At typical commercial inclusion levels (1 kg/t), the activity of a supplemental beta-glucanase, added to feed in a commercial mill was determined by averaging several measurements, with a precision of +/-4%, variation between individual readings of +/-11.3% (SD), and recovery of 109%. By using high-concentration feed extracts, the method was sensitive enough to detectbackground and(or) supplemental beta-glucanase activities as low as .05 kg/t supplement equivalent. This method allows consumers, producers, and regulatory authorities to measure the activity of beta-glucanasein feed at commercial inclusion levels and, hence, study the effects of processes such as pelleting and extrusion on such supplements.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 01:01:50