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Titolo:
EFFECTS OF POSTMORTEM AGING TIME, ANIMAL AGE, AND SEX ON DEGRADATION OF TITIN AND NEBULIN IN BOVINE LONGISSIMUS MUSCLE
Autore:
HUFFLONERGAN E; PARRISH FC; ROBSON RM;
Indirizzi:
IOWA STATE UNIV SCI & TECHNOL,DEPT ANIM SCI AMES IA 50011 IOWA STATE UNIV SCI & TECHNOL,DEPT ANIM SCI AMES IA 50011
Titolo Testata:
Journal of animal science
fascicolo: 4, volume: 73, anno: 1995,
pagine: 1064 - 1073
SICI:
0021-8812(1995)73:4<1064:EOPATA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM ACTIVATED FACTOR; GEL-ELECTROPHORESIS; SKELETAL-MUSCLE; MYOFIBRILLAR PROTEINS; MONOCLONAL-ANTIBODIES; ALPHA-CONNECTIN; SMOOTH-MUSCLE; TENDERNESS; STORAGE; BEEF;
Keywords:
BEEF; MYOFIBRILS; POSTMORTEM CHANGES; MEAT QUALITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
E. Hufflonergan et al., "EFFECTS OF POSTMORTEM AGING TIME, ANIMAL AGE, AND SEX ON DEGRADATION OF TITIN AND NEBULIN IN BOVINE LONGISSIMUS MUSCLE", Journal of animal science, 73(4), 1995, pp. 1064-1073

Abstract

This study was conducted to determine degradation of the giant myofibrillar proteins titin and nebulin in postmortem aged beef, with known tenderness values, from animals differing in sex (steers vs bulls) andage (cows vs steers and bulls). Ten bulls and 10 steers (both groups were approximately 14 mo old) and 10 cows (44 to 108 mo old) were slaughtered. Longissimus muscle samples were removed for determination of Warner-Bratzler shear force, sensory panel tenderness evaluation, and SDS-PAGE analysis at 3, 7, 14, and 28 d postmortem. The SDS-PAGE analysis of titin and nebulin revealed that titin often migrated as three closely-spaced bands (T-1, T-1-2, T-2, in increasing order of migration) in 3-d postmortem samples. With increasing time postmortem, intact titin (T-1) decreased and degraded titin (T-2) increased in all samples. Within a class (i.e., steers, bulls, or cows) the rate of conversionof T-1 to T-2 was slower in the less-tender samples. The T-1 to T-2 conversion postmortem was slower in the intact males (bulls) than in the castrated males (steers). The T-1 to T-2 conversion postmortem also was slower in the older animals (cows) in comparison to the younger steers, but not in comparison to the younger bulls. Nebulin was degradedby 3 d postmortem in tender samples from steers, but some nebulin remained in the less-tender 3-d samples from steers and in all of the 3-dsamples from bulls and older animals (cows). Intact nebulin was absent in all 7-d samples, regardless of the class of animal. Our results suggest that titin and nebulin are degraded at faster rates in more tender beef samples within each of the three classes of animals examined. The rate of degradation seems to differ when sex and age classifications are compared.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 02:31:46