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Titolo:
A REAGENTLESS AMPEROMETRIC ELECTRODE BASED ON CARBON-PASTE, CHEMICALLY-MODIFIED WITH D-LACTATE DEHYDROGENASE, NAD(-LACTIC ACID ANALYSIS .1.CONSTRUCTION, COMPOSITION, AND CHARACTERIZATION(), AND MEDIATOR CONTAINING POLYMER FOR D)
Autore:
SHU HC; MATTIASSON B; PERSSON B; NAGY G; GORTON L; SAHNI S; GENG L; BOGUSLAVSKY L; SKOTHEIM T;
Indirizzi:
LUND UNIV,CTR CHEM,DEPT ANALYT CHEM S-22100 LUND SWEDEN LUND UNIV,CTR CHEM,DEPT BIOTECHNOL S-22100 LUND SWEDEN MOLTECH CORP STONY BROOK NY 11794
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 3, volume: 46, anno: 1995,
pagine: 270 - 279
SICI:
0006-3592(1995)46:3<270:ARAEBO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTINAMIDE ADENINE-DINUCLEOTIDE; ELECTROCATALYTIC OXIDATION; CATION-EXCHANGER; BIOSENSORS; GLUCOSE; REGENERATION; OXIDASE; SAMPLES;
Keywords:
D-LACTATE; D-LACTATE DEHYDROGENASE; CARBON PASTE ELECTRODE; REDOX POLYMER; BIOSENSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
H.C. Shu et al., "A REAGENTLESS AMPEROMETRIC ELECTRODE BASED ON CARBON-PASTE, CHEMICALLY-MODIFIED WITH D-LACTATE DEHYDROGENASE, NAD(-LACTIC ACID ANALYSIS .1.CONSTRUCTION, COMPOSITION, AND CHARACTERIZATION(), AND MEDIATOR CONTAINING POLYMER FOR D)", Biotechnology and bioengineering, 46(3), 1995, pp. 270-279

Abstract

A reagentless carbon paste electrode was designed for D-lactic acid analysis in a flow injection system for the monitoring of the production of D-lactate in a batch fermentation. D-lactate dehydrogenase, nicotinamide adenine dinucleotide (NAD(+)), a synthetic redox polymer containing covalently attached toluidine blue O as mediator, graphite powder, and paraffin oil were used for the construction of the modified carbon paste electrode. D-Lactate selectivity was indicated by insignificant responses from a variety of possible interferences including L-lactate. The electrodes gave a linear response in the range between 0.05 and 5 mM D-lactate, with a detecting limit of 30 mu M, allowing a sample throughput of 20 h(-1). Preliminary investigations were made by covering the electrode surface with electropolymerized membranes. Satisfactory stability was observed, indicated by a reproducibility of 3.3% relative standard deviation (RSD, n = 31), with a non-membrane-covered electrode for the analysis of D-lactate in fermentation broth. A long-term stability (230 broth samples) was proven, suggesting the electrodes to have a good potential for use in on-line monitoring of fermentation processes. (C) 1995 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
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