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Titolo:
DISTINCT TPR MOTIFS OF CYC8 ARE INVOLVED IN RECRUITING THE CYC8-TUP1 COREPRESSOR COMPLEX TO DIFFERENTIALLY REGULATE PROMOTERS
Autore:
TZAMARIAS D; STRUHL K;
Indirizzi:
HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL BOSTON MA 02115
Titolo Testata:
Genes & development
fascicolo: 7, volume: 9, anno: 1995,
pagine: 821 - 831
SICI:
0890-9369(1995)9:7<821:DTMOCA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; GLUCOSE REPRESSION; BINDING PROTEIN; SNAP HELIX; TRANSCRIPTION; YEAST; GENE; PHOSPHOPROTEIN; EXPRESSION; MITOSIS;
Keywords:
TPR MOTIFS; CYC8(SSN6)-TUP1 COMPLEX; TRANSCRIPTIONAL REPRESSION; COREPRESSOR; EUKARYOTIC GENE REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
D. Tzamarias e K. Struhl, "DISTINCT TPR MOTIFS OF CYC8 ARE INVOLVED IN RECRUITING THE CYC8-TUP1 COREPRESSOR COMPLEX TO DIFFERENTIALLY REGULATE PROMOTERS", Genes & development, 9(7), 1995, pp. 821-831

Abstract

The yeast Cyc8(Ssn6)-Tup1 complex is required for transcriptional repression of distinct sets of genes that are regulated by glucose, oxygen, cell type, and DNA damage. It has been proposed that the Cyc8-Tup1 complex is a corepressor that is recruited to promoters by interactingwith pathway-specific DNA-binding proteins. Previously, we showed that a specific region of Tup1 mediates the general transcriptional repression function of the complex. Here, we define functional domains of Cyc8, a protein consisting primarily of 10 tandem copies of a TPR motif. Distinct combinations of TPR motifs are required specifically for direct interaction with Tup1, repression of oxygen-regulated genes, and repression of glucose-regulated genes. In contrast, the WD motifs of Tup1 are not essential for repression of genes regulated by glucose andoxygen, but they are required for those regulated by cell type and DNA damage. In addition, we show that the Cyc8-Tup1 complex functions both as a corepressor and an inhibitor of Mig1, a protein that binds to promoters of glucose-repressible genes. These observations suggest that different Cyc8 TPR motifs and the Tup1 WD domain mediate distinct protein-protein interactions that link the Cyc8-Tup1 corepressor to structurally dissimilar DNA-binding proteins required for pathway-specificregulation.

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Documento generato il 24/09/20 alle ore 05:19:15