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Titolo:
AN AUTORADIOGRAPHIC STUDY COMPARING THE INTERACTIONS OF 3-ALPHA-OH-5-ALPHA-PREGNAN-20-ONE, PREGNENOLONE SULFATE AND PENTOBARBITAL WITH [S-35] TBPS BINDING-SITES AND THEIR MODULATION BY GABA IN DIFFERENT STRUCTURES OF THE RAT-BRAIN
Autore:
VINCENS M; DARTOIS E; MOYSE E; HAOUR F; FILLION G;
Indirizzi:
UNIV PARIS 07,HOP LARIBOISIERE,2 RUE AMBROISE PARE F-75010 PARIS FRANCE INST PASTEUR F-75015 PARIS FRANCE
Titolo Testata:
Naunyn-Schmiedeberg's archives of pharmacology
fascicolo: 4, volume: 351, anno: 1995,
pagine: 356 - 362
SICI:
0028-1298(1995)351:4<356:AASCTI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINOBUTYRIC ACID(A) RECEPTORS; STEROID-HORMONE METABOLITES; SUBUNIT MESSENGER-RNAS; S-35 TBPS BINDING; A RECEPTOR; ALPHA-SUBUNIT; CHLORIDE IONOPHORE; RECOGNITION SITES; CHANNEL COMPLEX; BETA-SUBUNIT;
Keywords:
[S-35] TBPS BINDING SITES; 3-ALPHA-OH-5-ALPHA-PREGNAN-20-ONE; PREGNENOLONE SULFATE; PENTOBARBITAL GABA-(A) RECEPTOR COMPLEX; RAT BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
M. Vincens et al., "AN AUTORADIOGRAPHIC STUDY COMPARING THE INTERACTIONS OF 3-ALPHA-OH-5-ALPHA-PREGNAN-20-ONE, PREGNENOLONE SULFATE AND PENTOBARBITAL WITH [S-35] TBPS BINDING-SITES AND THEIR MODULATION BY GABA IN DIFFERENT STRUCTURES OF THE RAT-BRAIN", Naunyn-Schmiedeberg's archives of pharmacology, 351(4), 1995, pp. 356-362

Abstract

Using quantitative autoradiography, we have studied the distribution of the [S-35]-TBPS binding sites of the GABA-A receptor complex in various structures of the rat brain. High densities of binding sites wereobserved in layer IV of the cerebral cortex, in the globus pallidus, and in the thalamus. Intermediate densities of binding sites were observed in superficial and deep layers of the cerebral cortex, in the dentate gyrus and in the hippocampus. For all of these structures, the interactions of 3 alpha-OH-5 alpha-pregnan-20-one (3 alpha 5 alpha P), pregnenolone sulfate (PS) and pentobarbital with [S-35]-TBPS binding, in the presence or the absence of GABA were studied. In the absence of GABA, IC50 values for the inhibition of [S-35]-TBPS binding were 10(-6) M for 3 alpha 5 alpha P and 10(-4) M for PS and pentobarbital in allof the brain regions studied. In the presence of GABA (5 x 10(-6) M),IC50 values were decreased by one order of magnitude for 3 alpha 5 alpha P, PS, and pentobarbital in all structures studied except layer IVof the cortex, where the IC50 value for PS was more markedly decreased (up to two orders of magnitude). By contrast, IC50 values for picrotoxin and TBPS to inhibit [S-35]-TBPS binding were 10(-7) M and 10(-8) M, respectively, in the presence or absence of GABA.

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Documento generato il 06/04/20 alle ore 05:05:08