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Titolo:
GENETIC AND IMMUNOLOGICAL CHARACTERIZATION OF THE MICROSPORIDIAN SEPTATA-INTESTINALIS CALI, KOTLER AND ORENSTEIN, 1993 - RECLASSIFICATION TO ENCEPHALITOZOON-INTESTINALIS
Autore:
HARTSKEERL RA; VANGOOL T; SCHUITEMA ARJ; DIDIER ES; TERPSTRA WJ;
Indirizzi:
ROYAL TROP INST,KIT,DEPT BIOMED RES,MEIBERGDREEF 39 1105 AZ AMSTERDAMNETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,DEPT MED MICROBIOL 1105 AZ AMSTERDAM NETHERLANDS TULANE UNIV,DELTA REG PRIMATE RES CTR,DEPT MICROBIOL COVINGTON LA 70433
Titolo Testata:
Parasitology
, volume: 110, anno: 1995,
parte:, 3
pagine: 277 - 285
SICI:
0031-1820(1995)110:<277:GAICOT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA SEQUENCE; AIDS PATIENTS; N-SP; INFECTED PATIENTS; HELLEM; KERATOCONJUNCTIVITIS; DISSEMINATION; DIARRHEA; CUNICULI; CULTURE;
Keywords:
MICROSPORIDIA; ENCEPHALITOZOON; SEPTATA INTESTINALIS; POLYMERASE CHAIN REACTION; DIAGNOSIS; SMALL SUBUNIT RIBOSOMAL RNA; WESTERN BLOT IMMUNODETECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Hartskeerl et al., "GENETIC AND IMMUNOLOGICAL CHARACTERIZATION OF THE MICROSPORIDIAN SEPTATA-INTESTINALIS CALI, KOTLER AND ORENSTEIN, 1993 - RECLASSIFICATION TO ENCEPHALITOZOON-INTESTINALIS", Parasitology, 110, 1995, pp. 277-285

Abstract

The relationships between the Encephalitozoon-like Septata intestinalis and other microsporidia that occur in humans, notably Encephalitozoon cuniculi and Encephalitozoon hellem, is insufficiently documented using morphological descriptions alone. To assess mutual relationships,we have examined other phenotypic as well as genetic aspects of S. intestinalis, obtained both from tissue culture and clinical specimens, in comparison with a number of other microsporidia. Phenotypic characterization was performed by analysis of the protein composition and antigenic structure of Various microsporidian spores by SDS-PAGE and Western blotting. The genetic characterization consisted of the determination of the sequence of the S. intestinalis rrs gene encoding the smallsubunit ribosomal RNA (srRNA), restriction fragment length polymorphism (RFLP) analysis of amplified rrs genes and establishment of the degree of sequence identity between rrs genes of various microsporidian species. The unique sequence of rrs of S. intestinalis as well as the distinct RFLP and SDS-PAGE profiles indicate that S. intestinalis is clearly different from other human microsporidian species. However, its rrs gene shared about 90 % sequence identity with rrs of both Encephalitozoon spp., E. cuniculi and E. hellem. This is remarkably higher than the about 70 % identity observed between rrs of microsporidian species which belong to different genera and thus suggests that S. intestinalis should be regarded as a species of the genus Encephalitozoon. Western blots revealed a marked cross-reactivity between S. intestinalis and both species of Encephalitozoon which also stresses the close relationship between these organisms. It is concluded that S. intestinalisis so closely related to E. cuniculi, the type species of Encephalitozoon, that it should be reclassified as Encephalitozoon intestinalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 19:23:22