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Titolo:
PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN IRK-TYPE INWARD RECTIFIER K-CELL LINE( CHANNEL IN A TUMOR MAST)
Autore:
WISCHMEYER E; LENTES KU; KARSCHIN A;
Indirizzi:
MAX PLANCK INST BIOPHYS CHEM,AM FASSBERG 11 D-37077 GOTTINGEN GERMANY MAX PLANCK INST BIOPHYS CHEM D-37077 GOTTINGEN GERMANY
Titolo Testata:
Pflugers Archiv
fascicolo: 6, volume: 429, anno: 1995,
pagine: 809 - 819
SICI:
0031-6768(1995)429:6<809:PAMCOA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG HEART; RECTIFYING POTASSIUM CURRENT; MAGNESIUM BLOCK; DEPENDENT INACTIVATION; FUNCTIONAL EXPRESSION; VENTRICULAR CELLS; ANGIOTENSIN-II; PATCH-CLAMP; RECTIFICATION; MEMBRANE;
Keywords:
RBL CELLS; PATCH-CLAMP; G PROTEIN COUPLING; CHANNEL INHIBITION; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
E. Wischmeyer et al., "PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN IRK-TYPE INWARD RECTIFIER K-CELL LINE( CHANNEL IN A TUMOR MAST)", Pflugers Archiv, 429(6), 1995, pp. 809-819

Abstract

The basophilic leucaemia cell line RBL-2H3 exhibits a robust inwardlyrectifying potassium current, I-KIR, which is likely to be modulated by G proteins. We examined the physiological and molecular properties of this K-IR conductance to define the nature of the underlying channel species. The macroscopic conductance revealed characteristics typical of classical Kf inward rectifiers of the IRK type. Channel gating was rapid, first order (tau approximate to 1 ms at -100 mV) and steeply voltage dependent. Both activation potential and slope conductance were dependent on extracellular K+ concentration ([K+](o)) and inward rectification persisted in the absence of internal Mg2+. The current was susceptible to a concentration- and voltage-dependent block by extracellular Na+, Cs+ and Ba2+. Initial I-KIR whole-cell amplitudes as well as current rundown were dependent on the presence of 1 mM internal ATP. Perfusion of intracellular guanosine 5'-Q-(3-thiotriphosphate) (GTP[gamma S]) suppressed I-KIR with an average half-time of decline of approximately 400 s. It was demonstrated that the dominant IRK-type 25 pSconductance channel was indeed suppressed by 100 mu M preloaded GTP[gamma S]. Reverse transcriptase-polymerase chain reactions (RT-PCR) with RBL cell poly(A)(+) RNA identified a full length K+ inward rectifierwith 94% base pair homology to the recently cloned mouse IRK1 channel. It is concluded that RBL cells express a classical voltage-dependentIRK-type K+ inward rectifier RBL-IRK1 which is negatively controlled by G proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:30:20