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Titolo:
RECOMBINANT CHICKEN INTERFERON FROM ESCHERICHIA-COLI AND TRANSFECTED COS CELLS IS BIOLOGICALLY-ACTIVE
Autore:
SCHULTZ U; RINDERLE C; SEKELLICK MJ; MARCUS PI; STAEHELI P;
Indirizzi:
UNIV FREIBURG,DEPT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MED MICROBIOL & HYG,VIROL ABT D-79008 FREIBURG GERMANY UNIV CONNECTICUT,DEPT MOLEC & CELL BIOL STORRS CT 06269
Titolo Testata:
European journal of biochemistry
fascicolo: 1, volume: 229, anno: 1995,
pagine: 73 - 76
SICI:
0014-2956(1995)229:1<73:RCIFEA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE ACTIVATION; GAMMA; PURIFICATION; INDUCTION; GENES;
Keywords:
INTERFERON; CHICKEN; RECOMBINANT; ANTIVIRAL; ANTISERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
U. Schultz et al., "RECOMBINANT CHICKEN INTERFERON FROM ESCHERICHIA-COLI AND TRANSFECTED COS CELLS IS BIOLOGICALLY-ACTIVE", European journal of biochemistry, 229(1), 1995, pp. 73-76

Abstract

We have expressed a cDNA for virus-induced chick interferon in Escherichia coli. The product, a 19-kDa protein lacking the signal peptide, was purified to homogeneity from the bacterial inclusion bodies. Proteins in the insoluble fraction of bacterial lysates were dissolved in guanidine hydrochloride and subjected to chromatography on Q-Sepharose and MonoS columns. Purified recombinant chick interferon has a specific antiviral activity of approximately 10(8) IU/mg and is a powerful inducer of the interferon-responsive promoter of the chicken Mr gene. Culture medium of transfected COS cells expressing full-length chick interferon cDNA contained up to 5X10(4) IU antiviral activity/ml that could be neutralized by antibodies to purified recombinant chick interferon. The antibodies precipitated proteins of 23-28 kDa from the supernatants of transfected COS cells. Treatment with endoglycosidase F reduced the size of the immunoprecipitated proteins to approximately 20 kDa, demonstrating that chick interferon is a glycoprotein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:05:11