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Titolo:
REGULATION OF TRANSLOCATION OF THE DESMOYOKIN AHNAK PROTEIN TO THE PLASMA-MEMBRANE IN KERATINOCYTES BY PROTEIN-KINASE-C/
Autore:
HASHIMOTO T; GAMOU S; SHIMIZU N; KITAJIMA Y; NISHIKAWA T;
Indirizzi:
KEIO UNIV,SCH MED,DEPT DERMATOL,SHINJUKU KU,35 SHINANOMACHI TOKYO 160JAPAN KEIO UNIV,SCH MED,DEPT MOLEC BIOL,SHINJUKU KU TOKYO 160 JAPAN GIFU UNIV,SCH MED,DEPT DERMATOL GIFU 500 JAPAN
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 217, anno: 1995,
pagine: 258 - 266
SICI:
0014-4827(1995)217:2<258:ROTOTD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GENE AHNAK; CALCIUM-INDUCED REORGANIZATION; CULTURED HUMAN KERATINOCYTES; CELL-ADHESION MOLECULES; DESMOSOMAL PLAQUE; DESMOPLAKIN-II; GLYCOPROTEIN-3 DESMOCOLLINS; EPIDERMAL DESMOSOMES; CARCINOMA-CELLS; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
T. Hashimoto et al., "REGULATION OF TRANSLOCATION OF THE DESMOYOKIN AHNAK PROTEIN TO THE PLASMA-MEMBRANE IN KERATINOCYTES BY PROTEIN-KINASE-C/", Experimental cell research, 217(2), 1995, pp. 258-266

Abstract

Desmoyokin was identified as a desmosomal plaque protein. We previously demonstrated that desmoyokin is identical to a protein encoded by ahuman gene, AHNAK, whose expression is suppressed in neuroblastoma cells. Although this protein is distributed in the cytoplasm and the nucleus in various cells, it is associated closely with the plasma membrane in keratinocytes. In keratinocytes, desmoplakin translocates from the cytoplasm to the plasma membrane following both high calcium switchand protein kinase C (PKC) activation by 12-O-tetradecanoylphorbol-13-acetate (TPA). In the low calcium medium, the desmoyokin/AHNAK protein resides diffusely in the cytoplasm and the nucleus. However, 2 h after shift to the high calcium medium, the desmoyokin/AHNAK protein localized to the cell boundary in all cells in a pattern similar to that of desmoplakin. Selective PKC inhibitors completely inhibited the calcium-induced translocation of the desmoyokin/AHNAK protein, but the inhibition of desmoplakin translocation by these inhibitors was only partial. TPA also induced translocation of both the desmoyokin/AHNAK protein and desmoplakin, which was completely inhibited by PKC inhibitors. The calcium-induced phosphorylation of the desmoyokin/AHNAK protein wasconfirmed by immunoprecipitation using [P-32]orthophosphate-labeled keratinocytes. Furthermore, the study of extractability with non-ionic detergent indicated that desmoplakin, but not the desmoyokin/AHNAK protein, is associated with the cytoskeleton. These results suggested an involvement of PKC in the translocation of the desmoyokin/AHNAK protein in keratinocytes. It was, however, also suggested that different mechanisms are likely involved in the translocation of the desmoyokin/AHNAK protein and desmoplakin. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 06:38:53