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Titolo:
THE RELATIONSHIP BETWEEN EUKARYOTIC TRANSLATION AND MESSENGER-RNA STABILITY - A SHORT UPSTREAM OPEN READING FRAME STRONGLY INHIBITS TRANSLATIONAL INITIATION AND GREATLY ACCELERATES MESSENGER-RNA DEGRADATION INTHE YEAST SACCHAROMYCES-CEREVISIAE
Autore:
OLIVEIRA CC; MCCARTHY JEG;
Indirizzi:
GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS,MASCHERODER WEG 1 D-38124 BRAUNSCHWEIG GERMANY GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS D-38124 BRAUNSCHWEIG GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 15, volume: 270, anno: 1995,
pagine: 8936 - 8943
SICI:
0021-9258(1995)270:15<8936:TRBETA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA DECAY; SECONDARY STRUCTURE; NONSENSE MUTATIONS; CODING REGION; CODONS; ACID; AUG; REINITIATION; EFFICIENCY; RIBOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
C.C. Oliveira e J.E.G. Mccarthy, "THE RELATIONSHIP BETWEEN EUKARYOTIC TRANSLATION AND MESSENGER-RNA STABILITY - A SHORT UPSTREAM OPEN READING FRAME STRONGLY INHIBITS TRANSLATIONAL INITIATION AND GREATLY ACCELERATES MESSENGER-RNA DEGRADATION INTHE YEAST SACCHAROMYCES-CEREVISIAE", The Journal of biological chemistry, 270(15), 1995, pp. 8936-8943

Abstract

A new strategy was developed to study the relationship between the translation and degradation of a specific mRNA in the yeast Saccharomyces cerevisiae. A series of 5'-untranslated regions (UTR) was combined with the cat gene from the bacterial transposon Tn9, allowing us to test the influence of upstream open reading frames (uORFs) on translationand mRNA stability. The 5'-UTR sequences were designed so that the minimum possible sequence alteration, a single nucleotide substitution, could be used to create a 7-codon ORF upstream of the cat gene. The uORF was translated efficiently, but at the same time inhibited translation of the cat ORF and destabilized the cat mRNA. Investigations of various derivatives of the 5'-UTR indicated that cat translation was primarily attributable to leaky scanning of ribosomes past the uORF rather than to reinitiation. Therefore, these data directly demonstrate destabilization of a specific mRNA linked to changes in translational initiation on the same transcript. In contrast to the previously proposednonsense-mediated mRNA decay pathway, destabilization was not triggered by premature translational termination in the main ORF and was not discernibly dependent upon a reinitiation-driven mechanism. This suggests the existence of an as yet not described pathway of translation-linked mRNA degradation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:26:50