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Titolo:
2 TYPES OF VOLTAGE-GATED K-CHANNELS IN CAROTID-BODY CELLS OF ADULT CATS
Autore:
CHOU CL; SHIRAHATA M;
Indirizzi:
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT ENVIRONM HLTH SCI,615 N WOLFE ST BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT ENVIRONM HLTH SCI BALTIMORE MD 21205
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 742, anno: 1996,
pagine: 34 - 42
SICI:
0006-8993(1996)742:1-2<34:2TOVKI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTERIAL CHEMORECEPTOR CELLS; NEONATAL RAT; HYPOXIC SUPPRESSION; POTASSIUM CURRENTS; NATURAL STIMULI; IONIC CURRENTS; I CELLS; LOW PO2; OXYGEN; MATURATION;
Keywords:
CHEMORECEPTOR; GLOMUS CELL; HYPOXIA; K CURRENT; OXYGEN; SHEATH CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
C.L. Chou e M. Shirahata, "2 TYPES OF VOLTAGE-GATED K-CHANNELS IN CAROTID-BODY CELLS OF ADULT CATS", Brain research, 742(1-2), 1996, pp. 34-42

Abstract

The purpose of this study was to investigate if the oxygen-sensitive K channel is present in the carotid body cells of adult cats, and if all carotid body cells express the oxygen-sensitive K channel. A standard patch-clamp technique with a whole-cell configuration was applied to cultured carotid body cells from adult cats. The cells were continuously perfused with Krebs equilibrated with 5% CO2/air or 5% CO2/argon at room temperature. The results showed that electrophysiologically atleast two types of cells existed in cultured cat carotid body cells. One type expressed the oxygen sensitive K channel and the other expressed the oxygen-insensitive K channel. The oxygen-sensitive K channel was voltage-dependent with a threshold potential of -30 mV. No inactivation was observed during 30 ms of stimulation. The slope of the steady-state current-voltage curve was almost linear in the range from -30 mV to +50 mV. Hypoxia (pO(2) = 25 mmHg) reversibly depressed the K current by 22%. The current was inhibited by 4-aminopyridine (10 mM) and tetraethylammonium (4-25 mM), but insensitive to charybdotoxin (100 nM). The oxygen-insensitive K channel showed similar characteristics to that of the oxygen-sensitive K channel in the threshold and the speed of activation, and the shape of I-V curve. The cat is the third speciesin which the oxygen-sensitive K channel was found in the carotid body. The sensitivity of K channels to oxygen may be a unique feature of chemosensory cells, but the properties of the oxygen-sensitive K channels are different among cats, rats, and rabbits.

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Documento generato il 02/12/20 alle ore 17:58:14